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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: Nat Protoc. 2021 Jul 9;16(8):4004–4030. doi: 10.1038/s41596-021-00571-9
Component Per reaction
(μl)
Per 120 reactions
(μl)
Final concentration in 10-μl
reaction
10× CutSmart buffer 1 120
MspI (20 U/μl) 0.5 60 1 U/μl
(Double digest only) HaeIII (50 U/μl) (0.5) (60) (2.5 U/μl)
Total 1.5 (2) 180 (240)