Component | Per reaction (μl) |
Per 120 reactions (μl) |
Final concentration in 10-μl reaction |
---|---|---|---|
10× CutSmart buffer | 1 | 120 | 1× |
MspI (20 U/μl) | 0.5 | 60 | 1 U/μl |
(Double digest only) HaeIII (50 U/μl) | (0.5) | (60) | (2.5 U/μl) |
Total | 1.5 (2) | 180 (240) | — |