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Effects of overexpression of miR-21 on osteogenic differentiation of mouse BMSCs. (A) Observation of miR-21 expression in mouse BMSCs by qRT-PCR; (B) Detection of calcium nodule formation in BMSCs by alkaline phosphatase staining; (C) Manifestation of calcium nodule emergence in BMSCs by Alizarin red staining; (D) Detection of the expression degrees of osteogenesis-related genes Runx2, ALP, OCN and OPN in mouse BMSCs by qRT-PCR.
