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miR-21 negatively regulates PTEN expression. (A) The binding site of miR-21 and PTEN; (B) Luciferase reporter assay was used to detect the regulatory association between miR-21 and PTEN; (C) Detection of PTEN mRNA expression by qRT-PCR; (D) Detection of the protein expression of PTEN was observed by western blot; (E) Statistical results of western blot.
