Gene
|
Primers (5’ to 3’) |
Runx2
|
Upstream: AGAAGGCACAGACAGAAGCTTGA |
Downstream: AGGAATGCGCCCTAAATCACT |
ALP
|
Upstream: ACAACCTGACTGACCCTTCG |
Downstream: TCATGATGTCCGTGGTCAAT |
OCN
|
Upstream: TTCTGCTCACTCTGCTGACC |
Downstream: TTTGTAGGCGGTCTTCAAGC |
OPN
|
Upstream: ACACTTTCACTCCAATCGTCC |
Downstream: TGCCCTTTCCGTTGTTGTCC |
CEBPα
|
Upstream: GTGTGCACGTCTATGCTAAACCA |
Downstream: GCCGTTAGTGAAGAGTCTCAGTTTG |
CEBPβ
|
Upstream: TGGACAAGCTGAGCGACGAG |
Downstream: GAACAAGTTCCGCAGGGTGC |
FABP4
|
Upstream: TTCCTGTCGTCTGCGGTGATT |
Downstream: GATGCCTTTGTGGGAACCTGG |
PPARγ
|
Upstream: TCACAAGAGGTGACCCAATG |
Downstream: CCATCCTTCACAAGCATGAA |
β-actin
|
Upstream: ACTGCCGCATCCTCTTCCT |
Downstream: TCAACGTCACACTTCATGATGGA |