Fig. 1.

GSK-3484862 inhibitor treatment in mESC. A Brightfield images showing morphology of WT and TKO cells after six days of treatment with 0.1% DMSO or GSK-3484862 (2 µM, 20 µM and 200 µM) obtained from ChemieTek. Scale bar = 500 μm B Cell numbers after 30,000 WT or TKO mESC were treated with indicated culture conditions for six days. C Brightfield images of WT cells after 14 days of treatment with 0.1% DMSO, 2 µM or 10 µM of GSK-3484862 from ChemieTek or MedChemExpress Scale bar= 500 μm. D Graphs from two independent experiments show the cell growth of WT or TKO cells passaged and counted every 2–3 days