Fig. 3.

CircDLG1 promotes gastric cancer progression. a The relative expression of circDLG1 in gastric cancer cell lines and the gastric normal epithelial cell line GES-1 measured by qRT–PCR. b The expression of circDLG1 in HGC27 and SGC7901 cells treated with shRNAs (sh-circDLG1#NC, sh-circDLG1#1, sh-circDLG1#2, sh-circDLG1#3) measured by qRT–PCR. *P < 0.05, one-way ANOVA test for the comparison of the three groups, followed by Student’s t-test for the comparison of sh-1 and sh-1 group with sh-NC group, respectively; multigroup comparisons were adjusted using the Bonferroni method. The experiment was repeated three times. c CCK-8 assay of the gastric cancer cell lines HGC27 and SGC7901 treated with shRNAs. *P < 0.05, two-way ANOVA test, multigroup comparisons were adjusted using the Bonferroni method. The experiment was repeated three times. d Colony formation assays of the gastric cancer cell lines HGC27 and SGC7901 treated with shRNAs. *P < 0.05, one-way ANOVA test for the comparison of the three groups, followed by Student’s t-test for the comparison of sh-1 and sh-1 group with sh-NC group, respectively; multigroup comparisons were adjusted using the Bonferroni method. The experiment was repeated three times. Scale bar, 50 mm. e Transwell assay of the gastric cancer cell lines HGC27 and SGC7901 treated with shRNAs. *P < 0.05, one-way ANOVA test for the comparison of the three groups, followed by Student’s t-test for the comparison of sh-1 and sh-2 group with sh-NC group, respectively; multigroup comparisons were adjusted using the Bonferroni method. The experiment was repeated three times. Scale bar, 100 μm. f Immunofluorescence analysis of E-cadherin and N-cadherin expression after knockdown of circDLG1 in SGC7901 cells. Scale bar, 50 μm. g qRT–PCR analysis of epithelial and mesenchymal markers after knockdown of circDLG1 in SGC7901 cells. *P < 0.05, **P < 0.01, Student’s t-test; the experiment was repeated three times. h Sphere formation assay in SGC7901 and HGC27 cells after knockdown of circDLG1. *P < 0.05, **P < 0.01, Student’s t-test; the experiment was repeated three times. Scale bar, 100 μm. i qRT–PCR analysis of stemness-associated markers after knockdown of circDLG1 in SGC7901 cells. *P < 0.05, **P < 0.01, Student’s t-test; the experiment was repeated three times