Figure 2.

TBK1 recruitment to STING induces inflammation and bone erosion in DNase II^−/− mouse footpads. (A–C) H&E-stained joint tissues from hind footpads of 8-mo-old male mice (n = 5–8 in each genotype). (A) Representative images. Scale bars, 200 µm. (B and C) Blinded analyses of histologic scores calculated as described in Materials and methods (mean ± SD, n = 5–8 per genotype). (D) Representative images of TRAP-stained joint sections from 8-mo-old male mice. Scale bars, 200 µm. (E) TRAP scores calculated as described in Materials and methods (mean ± SD, n = 5–8 per genotype). For B, C, and E, each dot represents an individual mouse. Statistical analysis was done using a two-tailed, unpaired Student’s test. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The histologic scores were evaluated separately by two pathologists.