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. 2021 Dec 15;41(50):10222–10246. doi: 10.1523/JNEUROSCI.2154-20.2021

Table 3.

Summary of statistical analysis

Figure number Independent variables Statistical test Result Post hoc test Comparison groups Adjusted p value
Figure 1
Figure 1 B Diet × days Two-way ANOVA Diet × days
F(3,85) = 19.61, p < 0.0001;
Days F(3,85) = 28.6, p < 0.0001;
Diet F(1,85) = 143.7, p < 0.0001		 Sidak's multiple comparisons test, w1118 balanced vs sugar-reduced 0 d 0.9997
1 d <0.0001
2 d <0.0001
3 d <0.0001
Figure 1 C Pretreatment diet × consumption test diet Two-way ANOVA Pretreatment diet × consumption test diet
F(2,142) = 1.6,
p = 0.2054;
consumption diet
F(2,142) = 7.406,
p = 0.0009;
Pretreatment diet
F(1,142) = 1.936, p = 0.1662		 Sidak's multiple comparisons test, w1118 balanced vs w1118 sugar-reduced pretreatment Sugar-reduced consumption diet 0.6303
Balanced consumption diet 0.0792
Sugar-enriched consumption diet 0.9368
Figure 1 D Diet Mann–Whitney test w1118 balanced vs sugar-reduced 1 d Sucrose 0.0022
Fructose 0.0006
Glucose 0.0014
Figure 1 E Diet × days Two-way ANOVA Diet × days
F(7,126) = 6.363,
p < 0.00001;
Days
F(7,126) = 9.812,
p < 0.0001;
Diet
F(1,126) = 72.14, p < 0.0001		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched diet 0 d 0.9552
1 d 0.9999
2 d 0.8709
3 d 0.6033
4 d <0.0001
5 d 0.0003
6 d <0.0001
7 d <0.0001
Figure 1 F Pretreatment diet × consumption test diet Two-way ANOVA Pretreatment diet × consumption test diet
F(2,139) = 9.621,
p = 0.0001;
Consumption diet
F(2,139) = 0.6712,
p = 0.5127;
Pretreatment diet
F(1,139) = 7.691, p = 0.0063		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched pretreatment diet Sugar-reduced consumption diet 0.3549
Balanced consumption diet 0.135
Sugar-enriched consumption diet <0.0001
Figure 1 G Diet Mann–Whitney test w1118 balanced vs sugar-enriched diet, 4 d Sucrose 0.0238
Fructose 0.0022
Glucose 0.0022
Figure 2
Figure 2 B Diet × sucrose concentration Two-way ANOVA Diet × sucrose concentration
F(3,124) = 5.566,
p = 0.0013;
Sucrose concentration
F(3,124) = 108.8,
p < 0.0001;
Diet
F(1,124) = 37.05, p < 0.0001		 Sidak's multiple comparisons test, w1118 balanced vs sugar-reduced, 1 d 0 mm suc 0.9998
10 mm suc 0.0282
30 mm suc <0.0001
100 mm suc 0.0001
Figure 2 D Diet × sucrose concentration Two-way ANOVA Diet × sucrose concentration
F(3,156) = 3.428,
p = 0.0187;
Sucrose concentration
F(3,156) = 153.7,
p < 0.0001;
Diet
F(1,156) = 24.03, p < 0.0001		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched, 4 d 0 mm suc >0.9999
10 mm suc 0.0093
30 mm suc <0.0001
100 mm suc 0.1411
Figure 3
Figure 3 A Diet regimen × days Two-way ANOVA Diet regimen × days
F(3,67) = 4.309,
p = 0.0077;
Days
F(3,67) = 14.33,
p < 0.0001;
Diet regimen
F(1,67) = 15.18, p = 0.0002		 Sidak's multiple comparisons test, w1118 balanced → balanced vs sugar-reduced → balanced 0 d 0.9995
1 d <0.0001
2 d 0.03
3 d 0.9645
Figure 3 C Diet regimen × sucrose concentration Two-way ANOVA Diet regimen × sucrose concentration
F(15,348) = 4.555,
p < 0.0001;
Diet regimen
F(3,348) = 437.5,
p < 0.0001;
Sucrose concentration
F(5,348) = 26.17, p < 0.0001		 Tukey's multiple comparisons test, w1118 balanced 1 d vs sugar-reduced 1 d 0 mm suc >0.9999
10 mm suc 0.0004
30 mm suc <0.0001
100 mm suc <0.0001
Tukey's multiple comparisons test, w1118 balanced 2 d vs sugar-reduced 1 d + balanced 1 d 0 mm suc >0.9999
10 mm suc >0.9999
30 mm suc 0.01
100 mm suc 0.0007
Tukey's multiple comparisons test, w1118 balanced 3 d vs sugar-reduced 1 d + balanced 2 d 0 mm suc >0.9999
10 mm suc 0.286
30 mm suc 0.1972
100 mm suc 0.0264
Figure 3 D Diet regimen × days Two-way ANOVA Diet regimen × days
F(7,119) = 5.144,
p < 0.0001;
Days
F(7,119) = 8.939,
p < 0.0001;
Diet regimen
F(1,119) = 9.152, p = 0.003		 Sidak's multiple comparisons test, w1118 balanced → balanced vs sugar-enriched → balanced 1 d 0.9996
2 d 0.7448
3 d 0.3981
4 d <0.0001
5 d 0.9312
6 d 0.8033
7 d 0.9717
8 d 0.9814
Figure 3 F Diet regimen × sucrose concentration Two-way ANOVA Diet regimen × sucrose concentration
F(21,582) = 4.349,
p < 0.0001;
Sucrose concentration
F(3,582) = 496.5,
p < 0.0001;
Diet regimen
F(7,582) = 20.06, p < 0.0001		 Tukey's multiple comparisons test, w1118 balanced 4 d vs sugar-enriched 4 d 0 mm suc >0.9999
10 mm suc 0.0365
30 mm suc <0.0001
100 mm suc 0.4696
Tukey's multiple comparisons test, w1118 balanced 5 d vs sugar-enriched 4 d + balanced 1 d 0 mm suc >0.9999
10 mm suc 0.0266
30 mm suc <0.0001
100 mm suc <0.0001
Tukey's multiple comparisons test, w1118 balanced 6 d vs sugar-enriched 4 d + balanced 2 d 0 mm suc >0.9999
10 mm suc 0.5622
30 mm suc >0.9999
100 mm suc 0.3759
Tukey's multiple comparisons test, w1118 balanced 7 d vs sugar-enriched 4 d + balanced 3 d 0 mm suc >0.9999
10 mm suc 0.9963
30 mm suc >0.9999
100 mm suc 0.0843
Figure 4
Figure 4 A Diet Mann–Whitney test w1118 balanced vs sugar-reduced, 1 d 0.0043
Figure 4 B Diet × days Two-way ANOVA Diet × days
F(7,85) = 10.7,
p < 0.0001;
Days
F(7,85) = 9.944,
p < 0.0001;
Diet
F(7,85) =78.05, p < 0.0001		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched 0 d 0.9982
1 d 0.99
2 d 0.9998
3 d >0.9999
4 d 0.0003
5 d <0.0001
6 d <0.0001
7 d <0.0001
Figure 4 C Diet regimen × days Two-way ANOVA Diet regimen × days
F(6,70) = 2.462,
p = 0.0322;
Days
F(6,70) = 3.882,
p = 0.0021;
Diet regimen
F(1,70) = 10.98, p = 0.0015		 Sidak's multiple comparisons test, w1118 balanced → balanced vs sugar-enriched → balanced 1 d 0.8866
2 d 0.9938
3 d >0.9999
4 d 0.0018
5 d 0.0283
6 d 0.9994
7 d 0.9992
Figure 4 D Diet Mann–Whitney test balanced vs sugar-enriched, 4 d w 1118 0.0017
Ir76b 1 0.0851
Figure 4 E Diet × tastant Two-way ANOVA Diet × tastant
F(2,63) = 5.967,
p = 0.0042;
Tastant
F(2,63) = 110.7, p < 0.0001;
Diet
F(1,63) = 0.1558,
p = 0.6943		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched 4 d 100 mm suc 0.1164
3AA mix 0.0215
30 mm TCC >0.9999
Figure 5
Figure 5 A Diet × genotype Two-way ANOVA Diet × genotype
F(2,36) = 5.645,
p = 0.0074;
Genotype
F(2,36) = 29.4, p < 0.0001;
Diet
F(1,36) = 7.007,
p = 0.012		 Sidak's multiple comparisons test, balanced 1 d elav-GAL4 ctrl vs elav>Dop2R RNAi1 0.0719
elav-GAL4 ctrl vs elav>Dop2R RNAi2 0.0022
elav>Dop2R RNAi1 vs elav>Dop2R RNAi2 <0.0001
Sidak's multiple comparisons test, balanced vs sugar-reduced, 1 d elav-GAL4 ctrl 0.0008
elav>Dop2R RNAi1 0.3067
elav>Dop2R RNAi2 0.7995
Figure 5 B Diet × genotype Two-way ANOVA Diet × genotype
F(2,38) = 2.084,
p = 0.1384;
Genotype
F(2,38) = 8.539, p = 0.0009;
Diet
F(1,38) = 9.284,
p = 0.0042		 Sidak's multiple comparisons test, balanced, 1 d w1118 vs Dop2R1 0.3804
w1118 vs Dop2R2 0.9989
Dop2R1 vs Dop2R2 0.2273
Sidak's multiple comparisons test, balanced vs sugar-reduced, 1 d w 1118 0.0075
Dop2R 1 0.9423
Dop2R 2 0.3635
Figure 5 C Diet × genotype Two-way ANOVA Diet × genotype
F(2,28) = 7.765, p = 0.0021;
Genotype
F(2,28) = 19.98, p < 0.0001;
Diet
F(1,28) = 145.4,
p < 0.0001		 Sidak's multiple comparisons test, balanced, 4 d w1118 vs Dop2R1 0.2904
w1118 vs Dop2R2 0.3225
Dop2R1 vs Dop2R2 0.9999
Sidak's multiple comparisons test, balanced vs sugar-enriched, 4 d w 1118 0.0019
Dop2R 1 <0.0001
Dop2R 2 <0.0001
Figure 5 D Diet × genotype Two-way ANOVA Diet × genotype
F(2,35) = 3.487,
p = 0.0416;
Genotype
F(2,35) = 3.903, p = 0.0295;
Diet
F(1,35) = 16.26,
p = 0.0003		 Sidak's multiple comparisons test, balanced, 1 d w1118 vs Dop2R1 0.9987
w1118 vs Dop2R2 0.3036
Dop2R1 vs Dop2R2 0.1933
Sidak's multiple comparisons test, balanced vs sugar-reduced, 1 d w 1118 0.0002
Dop2R 1 0.0993
Dop2R 2 0.3417
Figure 7
Figure 7 A Diet × genotype Two-way ANOVA Diet × genotype
F(3,52) = 5.235, p = 0.0031;
Genotype
F(3,52) = 4.874, p = 0.0046;
Diet
F(1,52) = 72.05,
p < 0.0001		 Sidak's multiple comparisons test, balanced, 4 d w1118 vs dilp2 0.9986
w1118 vs dilp3 0.808
w1118 vs dilp5 0.0716
Sidak's multiple comparisons test, balanced vs sugar-enriched, 4 d w 1118 <0.0001
dilp2 <0.0001
dilp3 0.0035
dilp5 0.4748
Figure 7 B Diet × genotype Two-way ANOVA Diet × genotype
F(1,56) = 1.547, p = 0.2188;
Genotype
F(1,56) = 9.133, p = 0.038;
Diet
F(1,56) = 50.03,
p < 0.0001		 Sidak's multiple comparisons test, w1118 vs dilp5 Balanced 0.3738
Sugar-reduced 0.0084
Sidak's multiple comparisons test, balanced vs sugar-reduced, 1 d w 1118 <0.0001
dilp5 0.0008
Figure 7 E Diet × genotype Two-way ANOVA Diet × genotype
F(4,95) = 4.202, p = 0.0035;
Genotype
F(4,95) = 19.84, p < 0.0001;
Diet
F(1,95) = 56.16,
p < 0.0001		 Sidak's multiple comparisons test, balanced, 4 d dilp2-GAL4 vs UAS-dilp5 RNAi 0.6802
dilp2-GAL4 vs dilp2>dilp5 RNAi 0.9988
dilp2-GAL4 vs UAS-TNTG 0.0672
dilp2-GAL4 vs dilp2>TNTG 0.8096
UAS-dilp5 RNAi vs dilp2>dilp5 RNAi 0.9992
UAS-dilp5 RNAi vs dilp2>TNTG 0.217
UAS-TNTG vs dilp2>TNTG 0.6080
Sidak's multiple comparisons test, balanced vs sugar-enriched, 4 d dilp2-GAL4 <0.0001
UAS-dilp5 RNAi 0.0007
dilp2>dilp5 RNAi 0.4489
UAS-TNTG 0.0105
dilp2>TNTG 0.2572
Figure 7 F Diet × genotype Two-way ANOVA Diet × genotype
F(1,26) = 7.507e-006, p = 0.9978
Genotype
F(1,26) = 0.4644, p = 0.5016;
Diet
F(1,26) = 21.88,
p < 0.0001		 Sidak's multiple comparisons test, w1118 vs dilp5 Balanced 0.8703
Sugar-enriched 0.8612
Sidak's multiple comparisons test, balanced vs sugar-enriched, 4 d w 1118 0.0109
dilp5 0.0021
Figure 7 G Diet × genotype Two-way ANOVA Diet × genotype
F(1,26) = 24.27,
p < 0.0001;
Genotype
F(1,26) = 0.01833, p = 0.8933;
Diet
F(1,26) = 21.31,
p < 0.0001		 Sidak's multiple comparisons test, w1118 vs InR1/InR2 Balanced 0.0045
Sugar-enriched 0.0028
Sidak's multiple comparisons test, balanced vs sugar-enriched 4 d w 1118 <0.0001
InR 1 /InR 2 0.9753
Figure 7 H Diet × genotype Two-way ANOVA Diet × genotype
F(2,36) = 3.092,
p = 0.0576;
Genotype
F(2,36) = 9.274, p = 0.0006;
Diet
F(1,36) = 10.88,
p = 0.0002		 Sidak's multiple comparisons test, balanced 4 d UAS-InR RNAi vs elav-GAL4 0.0044
UAS-InR RNAi vs elav-GAL4>InR RNAi 0.8006
elav-GAL4 vs elav-GAL4>InR RNAi 0.0351
Sidak's multiple comparisons test, balanced vs sugar-enriched UAS-InR RNAi 0.0423
elav-GAL4 0.0031
elav-GAL4>InR RNAi 0.999
Figure 7 I Diet × genotype Two-way ANOVA Diet × genotype
F(6,83) = 5.527,
p < 0.0001;
Genotype
F(6,83) = 3.476, p = 0.0041;
Diet
F(1,83) = 179.4,
p < 0.0001		 Sidak's multiple comparisons test, balanced 4 d, genotype comparison UAS-InR RNAi vs v-Glut>InR RNAi 0.9987
UAS-InR RNAi vs Gad1>InR RNAi 0.9721
UAS-InR RNAi vs Ple>InR RNAi 0.9993
UAS-InR RNAi vs Trh>InR RNAi 0.0825
UAS-InR RNAi vs Tdc2>InR RNAi 0.9527
UAS-InR RNAi vs npF>InR RNAi 0.9845
Sidak's multiple comparisons test, balanced vs sugar-enriched 4 d UAS-InR RNAi <0.0001
v-Glut>InR RNAi <0.0001
Gad1>InR RNAi 0.0022
Ple>InR RNAi 0.0002
Trh>InR RNAi 0.8235
Tdc2>InR RNAi <0.0001
npF>InR RNAi <0.0001
Extended Data Figure 1
Extended Data Figure 1-1 Diet × days (preference) Two-way ANOVA Diet × days
F(3,85) = 1.169,
p = 0.3265;
Days
F(3,85) = 7.171,
p = 0.0002;
Diet
F(1,85) = 11.11, p = 0.0013		 Sidak's multiple comparisons test, w1118 balanced vs w1118 sugar-reduced 0 d 0.9922
1 d 0.4079
2 d 0.2306
3 d 0.0012
Extended Data Figure 1-1 Diet × days (participation) Two-way ANOVA Diet × days
F(3,85) = 2.911,
p = 0.0391;
Days
F(3,85) = 6.110,
p = 0.0008;
Diet
F(1,85) = 11.66, p = 0.0010		 Sidak's multiple comparisons test, w1118 balanced vs w1118 sugar-reduced 0 d >0.9999
1 d 0.7113
2 d 0.1281
3 d <0.0001
Extended Data Figure 1-2 Diet × days (participation) Two-way ANOVA Diet × days
F(3,85) = 0.2272,
p = 0.8772;
Days
F(3,85) = 6.110,
p = 0.0008;
Diet
F(1,85) = 0.2833, p = 0.0960		 Sidak's multiple comparisons test, w1118 balanced vs w1118 sugar-reduced 0 d >0.9999
1 d 0.507
2 d 0.7586
3 d 0.4953
Extended Data Figure 1-3 Diet × body part (weight) Two-way ANOVA Diet × body part (weight)
F(1,20) = 0.9544, p = 0.3403;
Body part (weight)
F(1,20) = 41.62,
p < 0.0001;
Diet
F(1,20) = 4.355,
p = 0.0499		 Sidak's multiple comparisons test, w1118 balanced vs sugar-reduced Head 0.0833
Body 0.6883
Extended Data Figure 1-4 Diet × days (preference) Two-way ANOVA Diet × days
F(7,126) = 4.646,
p = 0.1258;
Days
F(7,126) = 1.656,
p = 0.1258;
Diet
F(1,126) = 32.23, p < 0.0001		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched 0 d 0.9999
1 d >0.9999
2 d 0.0536
3 d >0.9999
4 d 0.1052
5 d 0.1807
6 d 0.025
7 d <0.0001
Extended Data Figure 1-4 Diet × days (participation) Two-way ANOVA Diet × days (participation)
F(7,126) = 0.8195,
p = 0.5728;
Days (participation)
F(7,126) = 2.521,
p = 0.0185;
Diet
F(1,126) = 1.417, p = 0.2361		 Sidak's multiple comparisons test, w1118 balanced diet vs sugar-enriched 0 d 0.9979
1 d 0.2761
2 d >0.9999
3 d >0.9999
4 d 0.9324
5 d >0.9999
6 d >0.9999
7 d 0.9876
Extended Data Figure 1-5 Diet × days
(participation)		 Two-way ANOVA Diet × days
F(7,116) = 0.8336, p = 0.5613;
Days
F(7,126) = 1.28,
p = 0.2655;
Diet
F(1,126) = 3.735,
p = 0.0555		 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched 0 d 0.1439
1 d 0.9922
2 d >0.9999
3 d 0.8270
4 d 0.9914
5 d >0.9999
6 d 0.9946
7 d >0.9999
Extended Data Figure 1-6 Diet × body part Two-way ANOVA F(1,24) = 9.469, p = 0.0052 Sidak's multiple comparisons test, w1118 balanced vs sugar-enriched Head 0.9662
Body 0.0008
Extended Data Figure 1-7, left Pretreatment condition One-way ANOVA F(2,12) = 7.588, p = 0.0074 Tukey's multiple comparisons test Balanced
(unstarved) vs sugar-reduced (unstarved)		 0.0394
Balanced
(starved) vs balanced (unstarved)		 0.6718
Sugar-reduced (unstarved) vs balanced (starved) 0.0114
Extended Data Figure 1-7, right Pretreatment condition One-way ANOVA F(2,15) = 66.59, p < 0.0001 Tukey's multiple comparisons test Balanced
(unstarved) vs sugar-enriched (unstarved)		 <0.0001
Balanced
(starved) vs balanced (unstarved)		 0.3368
Sugar-enriched (unstarved) vs balanced (starved) <0.0001
Extended Data Figure 2
Extended Data Figure 2-1 Diet pretreatment × sucrose concentration Two-way ANOVA Interaction
F(3,88) = 4.757,
p = 0.0040;
Sucrose concentration
F(3,88) = 109.1,
p < 0.0001;
Diet
F(1,88) = 5.967, p = 0.0166		 Sidak's multiple comparisons test, balanced vs sugar-reduced 0 mm suc >0.9999
10 mm suc 0.0212
30 mm suc 0.0069
100 mm suc 0.5976
Extended Data Figure 2-2 Diet pretreatment × sucrose concentration Two-way ANOVA Interaction
F(3,108) = 4.706,
p = 0.004;
Sucrose concentration
F(3,108) = 93.65,
p < 0.0001;
Diet
F(1,108) = 31.03, p < 0.0001		 Sidak's multiple comparisons test, balanced vs sugar-enriched 0 mm suc >0.9999
10 mm suc 0.001
30 mm suc <0.0001
100 mm suc 0.1023
Extended Data Figure 5
Extended Data Figure 5-1 Diet Independent samples t test Dop2R 0.324177
Extended Data Figure 5-3 Diet × genotype Two-way ANOVA Diet × genotype
F(2,38) = 0.3505, p = 0.7066;
Genotype
F(2,38) = 1.861,
p = 0.1694;
Diet
F(1,38) = 0.2966,
p = 0.5892		 Sidak's multiple comparisons test, balanced vs sugar-reduced, 1 d w 1118 >0.9999
Dop2R 1 >0.9999
Dop2R 2 0.6014
Extended Data Figure 5-4 Pretreated diet Log-rank test Df = 1, χ2 =
0.5312		 Mantel–Cox Water vs sugar-reduced 0.4661
Extended Data Figure 5-6 Genotype/pretreatment × sucrose concentration Two-way ANOVA Genotype/pretreatment × sucrose concentration
F(9,373) = 6.873, p < 0.0001;
Sucrose concentration
F(3,373) = 449.3,
p < 0.0001;
Genotype/pretreatment
F(3,373) = 32.83,
p < 0.0001		 Tukey's multiple comparisons test, w1118 balanced vs sugar-reduced, 1 d 0 mm suc 0.9969
10 mm suc 0.0017
30 mm suc <0.0001
100 mm suc <0.0001
Tukey's multiple comparisons test, w1118 balanced vs Dop2R1 balanced 0 mm suc 0.7828
10 mm suc 0.8215
30 mm suc 0.5909
100 mm suc 0.294
Tukey's multiple comparisons test, Dop2R1 balanced vs Dop2R1 sugar-reduced 0 mm suc >0.9999
10 mm suc >0.9999
30 mm suc 0.0244
100 mm suc 0.0002
Extended Data Figure 5-7 Diet × genotype Two-way ANOVA Diet × genotype
F(2,40) = 4.420, p = 0.0184;
Genotype
F(2,40) = 11.24,
p = 0.0001;
Diet
F(1,40) = 24.31,
p < 0.0001		 Sidak's multiple comparisons test, balanced 1 d UAS-Dop2R RNAi vs Gad1-GAL4>Dop2R RNAi 0.0428
UAS-Dop2R RNAi vs Gad1-GAL4 0.036
Gad1-GAL4>Dop2R RNAi vs Gad1-GAL4 0.9958
Sidak's multiple comparisons test, balanced vs sugar-reduced 1 d Dop2R RNAi 0.0303
Gad1-GAL4>Dop2R RNAi 0.7004
Gad1-GAL4 <0.0001
Extended Data Figure 5-9 Genotype (relative expression) Independent samples t test Dop2R 0.6521
Extended Data Figure 5-10 Diet × genotype (left) Two-way ANOVA Diet × genotype
F(2,49) = 1.054, p = 0.3564;
Genotype
F(2,49) = 7.388,
p = 0.0016;
Diet
F(1,49) = 63.32
p < 0.0001		 Sidak's multiple comparisons test, balanced, 1 d Gr64f-GAL4 vs UAS-D2R RNAi1 0.1064
Gr64f-GAL4 vs Gr64f>D2R RNAi1 0.381
UAS-Dop2R RNAi1 vs Gr64f>D2R RNAi1 0.946
Sidak's multiple comparisons test, balanced vs sugar-reduced, 1 d Gr64f-GAL4 <0.0001
UAS-D2R RNAi1 0.0057
Gr64f-GAL4>D2R RNAi1 <0.0001
Extended Data Figure 5-10 Diet × genotype (right) Two-way ANOVA Diet × genotype
F(7,97) = 3.41, p = 0.0027;
Genotype
F(7,97) = 4.784,
p = 0.0001;
Diet
F(1,97) = 158.9,
p < 0.0001		 Sidak's multiple comparisons test, balanced vs sugar-reduced 1 d Tdc2-GAL4>Dop2R RNAi 0.0128
Tdc2-GAL4 0.0001
Ple>Dop2R RNAi 0.0026
Ple-GAL4 <0.0001
Trh>Dop2R RNAi 0.0002
Trh-GAL4 0.0219
v-Glut>Dop2R RNAi <0.0001
v-Glut-GAL4 0.0276
Extended Data Figure 5-11 Diet × sucrose concentration Two-way ANOVA Diet × sucrose concentration
F(9,208) = 3.177, p = 0.0013;
Genotype
F(3,208) = 362.8,
p < 0.0001;
Diet
F(3,208) = 13.91,
p < 0.0001		 Tukey's multiple comparisons test, UAS-D2R RNAi balanced vs sugar-reduced 1 d 0 mm suc >0.9999
10 mm suc 0.0027
30 mm suc 0.0164
100 mm suc 0.0004
Tukey's multiple comparisons test, Gad1-GAL4>UAS-D2R RNAi balanced vs sugar-reduced 1 d 0 mm suc 0.9946
10 mm suc 0.0993
30 mm suc 0.2012
100 mm suc 0.1977
Extended Data Figure 6
Extended Data Figure 6-1 Diet (brain) Independent samples t test dilp3 0.680683
dilp5 0.009507
Extended Data Figure 6-1 Diet (proboscis) Independent samples t test dilp3 0.753426
dilp5 0.680805
Extended Data Figure 7
Extended Data Figure 7-2 Diet × genotype (participation) Two-way ANOVA Diet × genotype
F(3,52) = 0.05811, p = 0.9814;
Genotype
F(3,52) = 5.497,
p = 0.0023;
Diet
F(1,52) = 0.393,
p = 0.5335		 Sidak's multiple comparisons test, balanced vs sugar-enriched 4 d w 1118 >0.9999
dilp2 0.973
dilp3 0.9983
dilp5 0.9848
Extended Data Figure 7-3 Diet/pretreatment × sucrose concentration Two-way ANOVA Diet/pretreatment × sucrose concentration
F(9,379) = 7.609, p < 0.0001;
Sucrose concentration
F(3,379) = 428.8,
p < 0.0001;
Diet/pretreatment
F(3,379) = 34.27,
p < 0.0001		 Tukey's multiple comparisons test, w1118 balanced vs w1118 sugar-enriched 4 d 0 mm suc 0.9968
10 mm suc 0.03
30 mm suc <0.0001
100 mm suc 0.0653
Tukey's multiple comparisons test, dilp5 balanced vs dilp5 sugar-enriched 0 mm suc mm 0.9998
10 mm suc 0.0174
30 mm suc 0.9728
100 mm suc 0.0006
Tukey's multiple comparisons test, w1118 balanced vs dilp5 balanced 0 mm suc 0.9654
10 mm suc <0.0001
30 mm suc <0.0001
100 mm suc 0.0005
Extended Data Figure 7-4 Genotype (brain) Independent samples t test dilp5 0.360222
Extended Data Figure 7-5 Genotype (brain) Independent samples t test InR 0.070619

A summary of statistical analyses used in this study, which includes information about the statistical tests and p values.