FIGURE 1.

SR12343 reduces cellular senescence in both mouse and human cells. (a) Representative images of SA‐β‐gal senescence assay by C₁₂FDG staining in senescent Ercc1 ^−/− MEFs and IMR90 cells with or without treatment of SR12343 (50 µM), where blue fluorescence indicates DNA staining with Hoechst 33324 and green fluorescence indicates SA‐β‐gal staining with C₁₂FDG. Senescence was induced by oxidative stress (20% O₂, MEFs) and genotoxic stress (etoposide, IMR90). (b) Quantification of C₁₂FDG senescence assay. Error bars indicate SEM for n = 3. RT‐qPCR analysis of expression of senescence biomarkers in senescent (c) Ercc1 ^−/− MEFs and (d) IMR90 cells with or without treatment of SR12343 (50 µM). Error bars indicate SEM for n = 4