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. 2021 Nov 3;20(12):e13486. doi: 10.1111/acel.13486

FIGURE 6.

FIGURE 6

Chronic treatment of SR12343 reduces cellular senescence and improves pathologies in multiple tissues of old WT mice. (a) Wild‐type mice at 110 weeks old were treated intraperitoneally with vehicle or 30 mg/kg of SR12343 for 17 weeks before sacrifice for tissue analysis. RT‐qPCR analysis of the (b) liver and (c) lung of wild‐type old mice was performed to evaluate the expression of senescent markers and SASP factors. Error bars indicate SEM. Vehicle, n = 6; SR12343, n = 4. (d) Western blots showing expression of p‐p65/p65, p16, and p21 in the liver of wild‐type old mice treated with vehicle or SR12343. (e) Representative images of H&E and Masson's trichrome staining of liver sections of vehicle and SR12343 treated naturally aged mice. Images were taken at 10×. (f) RT‐qPCR analysis of quadriceps of wild‐type old mice was performed to evaluate the expression of senescent markers and SASP factors. Error bars indicate SEM. Vehicle, n = 6; SR12343, n = 4. (g) Representative images of immunofluorescence for laminin of muscle sections in vehicle and SR12343 treated naturally aged mice. (h) Distribution of cross‐sectional areas (CSAs) of muscle fibers. Error bars indicate SEM. n = 3 per group.