Fig. 3. Partial BLI sensor-grams for measuring the binding affinities of SARS-CoV-2 S protein to heparin. (a) Biotinylated heparin (0–181.8 nM) was immobilized to streptavidin biosensors which were then exposed to SARS-CoV-2 S protein (1 μM). (b) Plot of the BLI signal of the heparin-dose response of the binding affinity of SARS-CoV-2 S protein. (c) Binding kinetics was analyzed by fitting the 1 : 1 Langmuir model to evaluate the dissociation constant (Kd).
