FIG 4.

Small but significant differences in the representation of microbial taxa in the metaproteomes based on the preservation method. Bars represent the mean percent proteinaceous biomass for each taxon at the phylum level (A) or the genus level (B). Biomass contributions of specific taxa were calculated using the method described by Kleiner et al. (32). Error bars represent the standard deviation (n = 8, except for the ethanol treatment, for which n = 7). Asterisks represent statistical significance (t test, paired, two-tailed, P < 0.05). The eight most abundant genera are displayed in the figure. Percentages are low because genus-level taxonomy could be assigned for 11.1 ± 0.53% (n = 47) of the total proteinaceous biomass in our samples, distributed over 28 different microbial genera.