FIG 7.

Validation of RNA-seq data. RNA-seq expression levels indicated in TPM (transcripts per million) are shown in the top bar graphs. Error bars represent the mean and standard error of the biological replicates from all the strains used in this study (n = 9). For the qPCR, the relative expression of transcript levels was normalized to the corresponding level of the guanine nucleotide-binding protein transcript, and error bars represent the mean and standard error of quadruplicate samples performed twice from all the strains used in this study (bottom bar graphs). Reverse transcription-qPCR was performed according to MIQE guidelines.