TABLE 1.
Characteristics of EAV-specific MAbs
| MAba | Isotype | IgG concn (μg/ml) | ELISA ODb | Antibody titerc | Protein specificity | Neutralization (titer)d |
|---|---|---|---|---|---|---|
| E1/A19 | 2a | 3 | 2.6 (0.21) | 40 | N | − |
| E1/b5 | 1 | 20 | 3.3 (0.18) | 80 | N | − |
| E1/c4 | 1 | 5 | 2.0 (0.19) | 20 | N | − |
| E3/a9 | 1 | 5 | 3.2 (0.18) | 20 | N | − |
| E3/a61 | 1 | 9 | 3.2 (0.24) | 20 | N | − |
| E3/c16 | 2a | 42 | 1.9 (0.32) | 40 | GL | − |
| E3/d10 | 2b | 30 | 1.4 (0.31) | 10 | GL | − |
| E3/d17 | 2b | 9 | 3.3 (0.21) | 20 | N | − |
| E3/d52 | 2b | 12 | 2.4 (0.22) | 20 | N | − |
| E6/A3 | 2a | 22 | 0.6 (0.21) | 3 | GL? | +(80) |
| E6/A7 | 1 | 85 | 2.6 (0.21) | 160 | N | − |
| E6/a27 | 1 | 5 | 1.8 (0.18) | 10 | N | − |
| E6/B8 | 1 | 12 | 1.0 (0.28) | 10 | GL | − |
| E6/d24 | 1 | 4 | 2.8 (0.17) | 40 | N | − |
| E7/d15-c1 | 2b | 50 | 1.2 (0.32) | 20 | GL | +(40) |
| E7/d15-c9 | 2a | 42 | 1.7 (0.71) | 40 | GL | +(5) |
a
Hybridoma culture supernatant.
b
OD reading for EAV-infected (mock-infected) Vero cells that were fixed to the ELISA plate.
c
By IPMA as the reciprocal of the highest dilution of the culture supernatant at which specific cytoplasmic reactivity was clearly observed.
d
Reciprocal of the highest dilution of the culture supernatant that completely inhibited the CPE of 100 TCID50s of EAV.