FIG. 1.

Immunoblotting analysis of endogenous PKC subtypes in HeLa cells. Total cell lysates (25 μg) extracted from HeLa cells (lanes 1) were separated by sodium dodecyl sulfate–7.5% polyacrylamide gel electrophoresis, transferred onto nitrocellulose membranes, and stained with antibodies against each PKC subtype. Rat brain homogenate was used as a positive control for αPKC, δPKC, ɛPKC, and ζPKC, and recombinant ηPKC expressed in CHO-K1 cells was used as a positive control for ηPKC (lanes 2). The α, δ, and ζ subtypes of PKC were detected with reasonable molecular masses in HeLa cells. The results shown are representative of two independent experiments.