FIG. 3.

Characterization of the ceramide-induced translocation of δPKC-GFP. (A) C₂-dihydroceramide (DH-C₂-Cer) (10 μM), an inactive ceramide, failed to induce translocation of δPKC-GFP. (B) Preincubation with D609 (200 μg/ml) for 30 min did not affect the C₂-ceramide (C₂-Cer)-induced translocation of δPKC-GFP. (C) After translocation of δPKC-GFP to the perinuclear region by treatment with 10 μM C₂-ceramide for 20 min, 1 μM TPA irreversibly induced translocation of all the δPKC-GFP to the plasma membrane within 3 min. The results shown are representative of three independent experiments. Bars, 10 μm.