FIG. 4.
Ceramide-induced translocation of δPKC-GFP assessed by immunoblotting analysis. With immunoblotting analysis, δPKC-GFP was detected as a 110-kDa band that was more abundant in the cytosolic fraction (c). C2-ceramide treatment (10 μM, 20 min) induced translocation of δPKC-GFP from the cytosolic fraction to the particulate fraction (p). No degradation products were detected before or after treatment with C2-ceramide (left panel). The level of δPKC-GFP in the total homogenate was not changed after ceramide treatment for 20 min (right panel). The results shown are representative of three independent experiments.