FIG. 6.

Effects of IFN-γ on PKC subtype translocation in HeLa cells. (A) Treatment with IFN-γ (100 U/ml) induced translocation of δPKC-GFP from the cytoplasm to the perinuclear region within 5 min after treatment of HeLa cells in culture medium containing FBS (top row). The translocation of δPKC-GFP was not altered by eliminating FBS from the culture medium (bottom row). (B) IFN-γ (100 U/ml) did not affect the localization of αPKC-GFP expressed in HeLa cells. (C) IFN-γ (100 U/ml) did not affect the localization of ζPKC-GFP expressed in HeLa cells. The results shown are representative of three independent experiments. Bars, 10 μm.