FIG. 7.

Effects of sphingomyelinase inhibitors on IFN-γ-induced translocation of δPKC-GFP. (A) Treatment with Mg²⁺-free HEPES [Mg(−) HEPES] buffer containing 0.5 mM EDTA for 30 min blocked the IFN-γ (100 U/ml)-induced translocation of δPKC-GFP (top row). IFN-γ induced translocation of δPKC-GFP in normal HEPES buffer containing 1 mM Mg²⁺ (bottom row). (B) IFN-γ-induced translocation of δPKC-GFP was inhibited by pretreatment with 50 μM scyphostatin (Scypho.) for 15 min. However, scyphostatin did not inhibit the 10 μM C₂-ceramide (C₂-Cer)-induced translocation of δPKC-GFP (top row). GSH treatment (5 mM, 30 min) also abolished IFN-γ- but not C₂-ceramide-induced translocation of δPKC-GFP (bottom row). The results shown are representative of three independent experiments. Bars, 10 μm.