FIG. 8.

Effects of tyrosine kinase inhibitors on IFN-γ-induced translocation of δPKC-GFP. IFN-γ-induced translocation of δPKC-GFP was inhibited by pretreatment with 100 μM genistein for 30 min. However, genistein did not alter 10-μM ceramide (C₂-Cer)-induced translocation of δPKC-GFP (top row). Similarly, preincubation with tyrphostin AG490 (100 μM) for 30 min abolished IFN-γ-but not C₂-ceramide-induced translocation of δPKC-GFP (bottom row). The results shown are representative of three independent experiments. Bars, 10 μm.