FIG. 9.

Colocalization of δPKC-GFP and wheat germ agglutinin binding sites in δPKC-GFP-expressing HeLa cells treated with ceramide. HeLa cells transfected with δPKC-GFP were fixed after treatment with 10 μM C₂-ceramide for 20 min. Cells were treated with Texas red-conjugated wheat germ agglutinin (WGA) to make the Golgi complex visible. The localization of δPKC-GFP is shown in green (left). The Golgi complex is shown in red (center). On the merged image, overlapping GFP and Texas red signals appear yellow (right). The results are representative of four independent experiments. Bar, 10 μm.