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. Author manuscript; available in PMC: 2022 Jun 22.
Published in final edited form as: Biochemistry. 2021 Jun 7;60(24):1919–1925. doi: 10.1021/acs.biochem.1c00275

Table 2.

Stability (Tm °C) of M- and J-modified duplexes.a

ssRNAN (X) PNA7 (C) PNA8 (M) PNA9 (J)
ssRNA1 (G) 73.2 ± 0.5 59.1 ± 0.7 72.2 ± 0.6
ssRNA2 (A) 57.8 ± 0.4 55.4 ± 0.3 57.4 ± 0.5
ssRNA3 (C) 56.4 ± 0.4 56.8 ± 0.6 58.0 ± 0.4
ssRNA4 (U) 56.8 ± 0.3 55.8 ± 0.6 58.5 ± 0.5
ssDNAN (X) PNA7 (C) PNA8 (M) PNA9 (J)
ssDNA1 (G) 66.0 ± 0.7 45.3 ± 0.2 66.3 ± 0.5
ssDNA2 (A) 45.6 ± 0.5 41.5 ± 0.6 45.9 ± 0.4
ssDNA3 (C) 44.5 ± 0.3 46.5 ± 0.4 48.9 ± 0.3
ssDNA4 (T) 47.4 ± 0.3 45.6 ± 0.4 49.7 ± 0.2
a

Tm °C, average of five experiments ± stand. dev. measured at 260 nm and 2.5 μM of each ssRNAN or ssDNAN and PNA in the ITC buffer as in Table 1.