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. Author manuscript; available in PMC: 2021 Dec 15.

Published in final edited form as: Virology. 2021 May 10;560:17–33. doi: 10.1016/j.virol.2021.04.013

Fig 4. — The results shown are averages and standard deviations of the triplicate experiment. A) HMVEC were infected with ZIKV replicon with or without Gas6 (1 μg/ml) and NC dMLD R801 (30 μg/ml). B) ZIKV replicon was incubated with various concentrations of ZKA64 or sTIM1dMLD R801, and HMVEC were infected with the incubated virus in the presence of Gas6 (1 μg/ml). C) ZIKV replicon was incubated with 1 or 10 μg/ml of ZKA64 or sTIM1dMLDR801, or control medium. 293T, TIM-4 293T, DC-SIGN 293T, or CD300A 293T cells were infected with the incubated virus for 2 hours, and infections were analyzed by flow cytometry 24 hours post-infection. The MOI used for the experiments shown in Fig 4B and C are 0.02 and 0.03, respectively. Significance was calculated by comparing TIM-4, CD300A, or DC-SIGN 293T cell infection without blocking reagents to those with blocking reagents, using a two-sample two-sided unpaired student t-test (**, p<0.01).

Fig 4. — The results shown are averages and standard deviations of the triplicate experiment. A) HMVEC were infected with ZIKV replicon with or without Gas6 (1 μg/ml) and NC dMLD R801 (30 μg/ml). B) ZIKV replicon was incubated with various concentrations of ZKA64 or sTIM1dMLD R801, and HMVEC were infected with the incubated virus in the presence of Gas6 (1 μg/ml). C) ZIKV replicon was incubated with 1 or 10 μg/ml of ZKA64 or sTIM1dMLDR801, or control medium. 293T, TIM-4 293T, DC-SIGN 293T, or CD300A 293T cells were infected with the incubated virus for 2 hours, and infections were analyzed by flow cytometry 24 hours post-infection. The MOI used for the experiments shown in Fig 4B and C are 0.02 and 0.03, respectively. Significance was calculated by comparing TIM-4, CD300A, or DC-SIGN 293T cell infection without blocking reagents to those with blocking reagents, using a two-sample two-sided unpaired student t-test (**, p<0.01).