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. Author manuscript; available in PMC: 2021 Dec 15.

Published in final edited form as: Virology. 2021 May 10;560:17–33. doi: 10.1016/j.virol.2021.04.013

Fig 5. — Replication-competent ZIKV produced by mammalian cells (A) or mosquito cells (A), DENV (B), and EBOV GP pseudotyped lentiviral vector [EBOV GP (FUGW)] (B) were incubated with 1 or 10 μg/ml of ZKA64 or sTIM1dMLDR801, or control medium for 1 hour at room temperature. 293T and TIM-1 293T cells were infected with the incubated virus for 2 hours, and the infections were analyzed by flow cytometry 24 hours (ZIKV and DENV) or 72 hours [EBOV GP (FUGW)] post-infection. The results shown are averages and standard deviations of the triplicate experiment. Significance was calculated by comparing TIM-1 293T cell infection without blocking reagents to those with blocking reagents, using a two-sample two-sided unpaired student t-test (*, p<0.05; **, p<0.01). (C) Quantitation of the EGFP-labeled EBOV GP pseudotype binding to 293T cells and TIM-1 293T cells. The values of the Y-axis correspond to the MFI of EGFP signals, which increase by binding of EGFP-labeled virus. This experiment was repeated once in singlicate and twice in triplicate, and the results shown are averages and standard deviations of one representative triplicate experiment. Significance was calculated by comparing binding of the EBOV GP pseudotype to TIM-1 293T cells with or without blocking reagents, using a two-sample two-sided unpaired student t-test (**, p<0.01).

Fig 5. — Replication-competent ZIKV produced by mammalian cells (A) or mosquito cells (A), DENV (B), and EBOV GP pseudotyped lentiviral vector [EBOV GP (FUGW)] (B) were incubated with 1 or 10 μg/ml of ZKA64 or sTIM1dMLDR801, or control medium for 1 hour at room temperature. 293T and TIM-1 293T cells were infected with the incubated virus for 2 hours, and the infections were analyzed by flow cytometry 24 hours (ZIKV and DENV) or 72 hours [EBOV GP (FUGW)] post-infection. The results shown are averages and standard deviations of the triplicate experiment. Significance was calculated by comparing TIM-1 293T cell infection without blocking reagents to those with blocking reagents, using a two-sample two-sided unpaired student t-test (*, p<0.05; **, p<0.01). (C) Quantitation of the EGFP-labeled EBOV GP pseudotype binding to 293T cells and TIM-1 293T cells. The values of the Y-axis correspond to the MFI of EGFP signals, which increase by binding of EGFP-labeled virus. This experiment was repeated once in singlicate and twice in triplicate, and the results shown are averages and standard deviations of one representative triplicate experiment. Significance was calculated by comparing binding of the EBOV GP pseudotype to TIM-1 293T cells with or without blocking reagents, using a two-sample two-sided unpaired student t-test (**, p<0.01).