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. Author manuscript; available in PMC: 2021 Dec 15.

Published in final edited form as: Virology. 2021 May 10;560:17–33. doi: 10.1016/j.virol.2021.04.013

Fig. 7. — The results shown are averages and standard deviations of the triplicate experiment. A) Inhibition of single-cycle infection of Vero cells with ZIKV replicon by various concentrations of sTIM1dMLDR801 or ZKA64. B) Production of replication-competent ZIKV production at 1 and 2 days post-infection. Replication-competent ZIKV was incubated with 700 ng/ml of ZKA64 or sTIM1dMLDR801 for 30 min at 37°C. Vero cells were infected with the virus (MOI 0.01) preincubated with or without ZKA64, sTIM1dMLD R801, or heat-inactivated virus for 2 hours at 37°C. The cells were then washed 3 times with culture medium and cultured in fresh culture medium containing the same reagents (700 ng/ml) used for incubation with the virus. ZKA64 and sTIM1dMLDR801 were present days 0–6 post-infection at 700 ng/ml. Significance was calculated by comparing Vero cell infection without blocking reagents to those with blocking reagents, using a two-sample two-sided unpaired student t-test (**, p<0.01). C) Production of replication-competent ZIKV production from day 1 to day 6 post-infection.

Fig. 7. — The results shown are averages and standard deviations of the triplicate experiment. A) Inhibition of single-cycle infection of Vero cells with ZIKV replicon by various concentrations of sTIM1dMLDR801 or ZKA64. B) Production of replication-competent ZIKV production at 1 and 2 days post-infection. Replication-competent ZIKV was incubated with 700 ng/ml of ZKA64 or sTIM1dMLDR801 for 30 min at 37°C. Vero cells were infected with the virus (MOI 0.01) preincubated with or without ZKA64, sTIM1dMLD R801, or heat-inactivated virus for 2 hours at 37°C. The cells were then washed 3 times with culture medium and cultured in fresh culture medium containing the same reagents (700 ng/ml) used for incubation with the virus. ZKA64 and sTIM1dMLDR801 were present days 0–6 post-infection at 700 ng/ml. Significance was calculated by comparing Vero cell infection without blocking reagents to those with blocking reagents, using a two-sample two-sided unpaired student t-test (**, p<0.01). C) Production of replication-competent ZIKV production from day 1 to day 6 post-infection.