Fig. 5.

Proteomic profiles of glioma extracellular vesicles (EVs). A, Numbers of proteins detected in EVs from cultured cells of four glioblastomas by differential quantitative proteomics. B, Proteins detected in at least three of four samples each of EVs, cells, and original tumors. C, Unsupervised clustering based on 270 proteins present in all three sample types. D, Pearson correlation analysis of the proteomes of EVs, cells, (C) and tumor tissue (T). E, Overlap between proteins presents in at least three of four white matter (WM) or glioblastoma (GBM) samples. F, Unsupervised hierarchical clustering based on proteins (2172) detected in GBMs and WM. G, Proteins either exclusively detected in GBM tissue, upregulated in GBMs vs WM (≥2-fold), present in ≥3EV samples and in ≥3 GBMs.