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CAC3 overexpression suppresses the RAS/cAMP signal transduction pathway between RAS and PKA. The heat shock resistance of (A) isogenic wild-type (YJB195), RAS2G19V (YJB2235), and RAS2G19V CAC3-over expressing (OE) (YJB2320) strains and (B) isogenic wild-type (YJB1583), bcy1Δ (YJB2697), and bcy1Δ CAC3-overexpressing (YJB2781) strains was determined by incubation at 55°C for the indicated time periods as described in Materials and Methods.
