FIG. 2.

CAC1 and CAC2 are not required for CAC3 to suppress the RAS/cAMP pathway. The heat shock sensitivity of (A) cac1-1 (YJB1358), RAS2^G19V cac1-1 (YJB2237), RAS2^G19V cac1-1 CAC3-overexpressing (OE) (YJB2322) or (B) cac2Δ (YJB1599), RAS2^G19V cac2Δ (YJB3563), and RAS2^G19V cac2Δ CAC3-overexpressing (YJB3876) was determined as described for Fig. 1. (C) Cells carrying Cac3p-GFP (YJB4034) were stained with DAPI and analyzed by fluorescence microscopy to observe both GFP (top panel) and DNA (middle panel). The merged image (bottom panel) shows that Cac3p-GFP localizes throughout the cytoplasm and nucleus. Darker regions correspond to the vacuoles.