FIG. 5.

CAC3 and NPR1 are mutally antagonistic. (A) CAC3 overexpression suppresses pseudohyphal growth. Σ1278B strains carrying either pGalSet (YJB3514) or pGalSet-CAC3 (YJB3515) were grown in either galactose (to induce CAC3 expression) or glucose (to repress CAC3 expression). The cells were then plated on either SLAD or SLADG medium to induce pseudohyphal growth either with or without CAC3 expression. Representative colonies were photographed after 3 days of growth at 30°C. For comparison, wild-type (YJB5724) and npr1/npr1 (YJB5723) Σ1278B strains are also shown. (B) NPR1 overexpression decreases telomeric silencing. Serial dilutions (1:10) of wild-type (YJB3610), cac3 (YJB1786), and pGalSET-NPR1 (YSJ328) strains were plated on either complete medium or medium with 5-fluoroorotic acid (FOA). Yeast cells were grown in medium containing either glucose (gluc) or galactose (gal), as indicated. Plates were photographed after 2 days of growth at 30°C.