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Overexpression of polyubiquitin (UBI4) suppresses the RAS2G19V-induced heat shock sensitivity. The heat shock resistance of RAS2G19V (YJB2235), RAS2G19V UBI4-overexpressing (OE) (YJB27334), RAS2G19V npr1Δ (YJB3723), and RAS2G19V npr1 UBI4-overexpressing (YJB5522) strains in the presence and absence of NPR1 and UB14 overexpression were determined as for Fig. 1.
