TABLE 1.
Yeast strains used
| Isogenic group and strain | Relevant characteristic(s) | Source or reference | |
|---|---|---|---|
| W303 | |||
| YJB195 | MATaura3-1 ade2-1 his3-11 leu2-3,112 can1-100 trp1-1 | Berman lab | |
| YJB209 | MATα ura3-1 ade2-1 his3-11 leu2-3,112 can1-100 trp1-1 | Berman lab | |
| YJB334 | MATa/α ura3-1/ura3-1 ade2-1/ ade2-1 his3-11/his3-11 leu2-3,112/leu2-3,112 can1-100/can1-100 trp1-1/trp1-1 | Berman lab | |
| YJB1358 | YJB209 cac1-1 | 11 | |
| YJB1599 | YJB209 cac2::TRP1 | 24 | |
| YJB1786 | YJB209 cac3::hisG VIIL::URA3-TEL | This study | |
| YJB2235 | YJB195 URA3::RAS2G19V | This study | |
| YJB2237 | YJB209 cac1-1 URA3::RAS2G19V | This study | |
| YJB2320 | YJB195 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2322 | YJB209 cac1-1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2543 | YJB195 sir3::TRP1 | Berman lab | |
| YJB2546 | YJB195 gcn5::URA3 | Berman lab | |
| YJB3135 | YJB195 gcn5::ura3::TRP1 URA3::RAS2G19V | This study | |
| YJB3141 | YJB195 gcn5::ura3::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB3554 | YJB195 npr1::LEU2 bcy1::URA3 | This study | |
| YJB3563 | YJB209 cac2::TRP1 URA3::RAS2G19V | This study | |
| YJB3610 | YJB209 VIIL::URA3-TEL pGalSET | This study | |
| YJB3670 | YJB195 sch9::TRP1 YCP50-RAS2G18A G19V | 50a | |
| YJB3674 | YJB195 sch9::TRP1 YCP50-RAS2G18A G19V YEP55-CAC3 | This study | |
| YJB3712 | YJB195 npr1::LEU2 | This study | |
| YJB3721 | YJB334 ura3-1/URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB3723 | YJB195 npr1::LEU2 URA3::RAS2G19V | This study | |
| YJB3724 | YJB2235 pCUP1-myc-UB14 | This study | |
| YJB3737 | YJB334 ura3-1/URA3::RAS2G19V | This study | |
| YJB3739 | YJB334 YEp55-CAC3 | This study | |
| YJB3763 | YJB195 sir3::TRP1 URA3::RAS2G19V | This study | |
| YJB3773 | YJB195 sir3::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB3785 | MATahis3 Leu2-3, 112 trp1 ura3-1 | 4 | |
| YJB3791 | YJB334 ura3-1/URA3::RAS2G19V npr1::LEU2/npr1::LEU2 | This study | |
| YJB3792 | YJB334 npr1::LEU2/npr1::LEU2 | This study | |
| YJB3863 | YJB195 URA3::RAS2G19V pGalSET-NPR1 | This study | |
| YJB3875 | YJB195 URA3::RAS2G19V pGalSET-NPR1 pGalSET-CAC3 | This study | |
| YJB3876 | YJB209 cac2::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB3896 | YJB3785 pGFP-RAS2 | This study | |
| YJB3897 | YJB3785 npr1::LEU2 pGEP-RAS2 | This study | |
| YJB3898 | YJB3785 YEp55-CAC3 pGFP-RAS2 | This study | |
| YJB3954 | YJB195 pGalSET-NPR1 | This study | |
| YJB4034 | YJB3785 YEp55-CAC3 | This study | |
| YJB4317 | YJB4034 YEp55-CAC3-GEP | This study | |
| YJB4506 | YJB4317 YEp55-CAC3-GFP [rho−] | This study | |
| YJB4632 | YJB209 gpa2::TRP1 | 50a | |
| YJB4635 | YJB209 gpr1::HIS3 | 50a | |
| YJB5235 | YJB195 URA3::RAS2G19V gpa2::TRP1 YEp55-CAC3 | This study | |
| YJB5243 | YJB195 URA3::RAS2G19V gpa2::TRP1 | 50a | |
| YJB5237 | YJB195 URA3::RAS2G19V gpr1::HIS3 YEp55-CAC3 | This study | |
| YJB5245 | YJB195 URA3::RAS2G19V gpr1::HIS3 | 50a | |
| YJB5522 | YJB3723 pCUP1-myc-UB14 | This study | |
| YJB5523 | YJB3737 pCUP1-myc-UB14 | This study | |
| YSJ328 | YJB3954 VIIL::URA3-TEL | This study | |
| Y2799 | MATα ura3-1 ade2-1 his3-11 leu2-3,112 can1-100 trp1-1 npr1::LEU2 pRS424 pGalSET-CAC3 | This study | |
| Y2800 | MATα ura3-1 ade2-1 his3-11 leu2-3,112 can1-100 trp1-1 npr1::LEU2 pRS424- 3HA-NPR1 pGalSET-CAC3 | This study | |
| Y2801 | MATα ura3-1 ade2-1 his3-11 leu2-3,112 can1-100 trp1-1 npr1::LEU2 pRS424-3HA- NPR1-KD pGalSET-CAC3 | This study | |
| YPH49/50 | |||
| YJB1583 | MATaura3-52 lys2-801 ade2-101 trp1-ars1 his3-200 leu2Δ1 | 31 | |
| YJB1584 | YJB1583 hat1::HIS3 | 31 | |
| YJB1585 | YJB1583 hat2::TRP1 | 31 | |
| YJB1586 | YJB1583 hat1::HIS3 hat2::TRP1 | 31 | |
| YJB2349 | YJB1583 URA3::RAS2G19V | This study | |
| YJB2350 | YJB1583 hat1::HIS3 URA3::RAS2G19V | This study | |
| YJB2366 | YJB1583 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2367 | YJB1583 hat1::HIS3 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2648 | YJB1583 hat2::TRP1 URA3::RAS2G19V | This study | |
| YJB2658 | YJB1583 hat2::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2672 | YJB1583 hat1::HIS3 hat2::TRP1 URA3::RAS2G19V | This study | |
| YJB2673 | YJB1583 hat1::HIS3 hat2::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2697 | YJB1583 bcy1::URA3 | This study | |
| YJB2781 | YJB1583 bcy1::URA3 YEp55-CAC3 | This study | |
| YDS3 | |||
| YJB2431 | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | 35a | |
| YJB2432 | YJB2431 hda1::TRP1 | 35a | |
| YJB2433 | YJB2431 rpd3::LEU2 | 35a | |
| YJB2434 | YJB2431 hda1::TRP1 rpd3::LEU2 | 35a | |
| YJB2474 | YJB2431 URA3::RAS2G19V | This study | |
| YJB2475 | YJB2431 hda1::TRP1 URA3::RAS2G19V | This study | |
| YJB2476 | YJB2431 rpd3::leu2::HIS3 URA3::RAS2G19V | This study | |
| YJB2474 | YJB2431 hda1::TRP1 rpd3::leu2::HIS3 URA3::RAS2G19V | This study | |
| YJB2529 | YJB2431 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2530 | YJB2431 rpd3::leu2::HIS3 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2531 | YJB2431 hda1::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB2532 | YJB2431 hda1::TRP1 rpd3::leu2::HIS3 URA3::RAS2G19V YEp55-CAC3 | This study | |
| HF7c | |||
| YJB1325 | MATaura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu2-3,112 gal4-542 gal180-538 LYS2::GAL1-HIS3 | ||
| URA3::(GAL4 17-mers)3-CYC1-lacZ | Clontech Inc. | ||
| YJB3197 | YJB1325 pGBT9-Cac3p pGAD1-CAC1 | This study | |
| YJB3438 | YJB1325 pGBT9-Cac3p pGAD1-NPR1561–605 | This study | |
| L1356 | |||
| YJB2136 | MATaura3-52 his3-200 leu2-1 met8-1 ilv1-1 trp1-901 cac3-878 lys2::his3Δ4 | 33 | |
| YJB2137 | YJB2136 hir3::LEU2 | 33 | |
| YJB2998 | YJB2136 hir3::leu2::TRP1 URA3::RAS2G19V | This study | |
| YJB2999 | YJB2136 hir3::leu2::TRP1 URA3::RAS2G19V YEp55-CAC3 | This study | |
| YJB3016 | YJB2136 URA3::RAS2G19V | This study | |
| YJB3017 | YJB2136 URA3::RAS2G19V YEp55-CAC3 | This study | |
| Σ1278b | |||
| YJB2444 | MATa/α ura3-52/ura3-52 | 24a | |
| YJB3514 | YJB2444 pGalSET | This study | |
| YJB3515 | YJB2444 pGalSET-CAC3 | This study | |
| YJB5723 | YJB5724 npr1::LEU2/npr1::LEU2 | 26 | |
| YJB5724 | MATa/α ura3-52/ura3-52 | 26 |