TABLE 2.
Effect of CAC3 overexpression on RAS/cAMP pathway activation in different mutant strainsa
| Notation type | Strain | Relevant genotype | Heat shock response |
|---|---|---|---|
| RAS/cAMP pathway | YJB195 | Wild type | Resistant |
| YJB2235 | RAS2G19V | Sensitive | |
| YJB2320 | RAS2G19V YEp55-CAC3 | Resistant | |
| YJB2697 | bcy1Δ | Sensitive | |
| YJB2781 | bcy1Δ YEp55-CAC3 | Sensitive | |
| Chromatin assembly | YJB2237 | RAS2G19V cac1-1 | Sensitive |
| YJB2322 | RAS2G19V cac1-1 YEp55-CAC3 | Resistant | |
| YJB3563 | RAS2G19V cac2Δ | Sensitive | |
| YJB3876 | RAS2G19V cac2Δ YEp55-CAC3 | Resistant | |
| Histone acetyltransferases | YJB2350 | RAS2G19V hat1Δ | Sensitive |
| YJB2367 | RAS2G19V hat1Δ YEp55-CAC3 | Resistant | |
| YJB2648 | RAS2G19V hat2Δ | Sensitive | |
| YJB2658 | RAS2G19V hat2Δ YEp55-CAC3 | Resistant | |
| YJB2672 | RAS2G19V hat1Δ hat2Δ | Sensitive | |
| YJB2673 | RAS2G19V hat1Δ hat2Δ YEp55-CAC3 | Resistant | |
| YJB3135 | RAS2G19V gcn5Δ | Sensitive | |
| YJB3141 | RAS2G19V gcn5Δ YEp55-CAC3 | Resistant | |
| Histone deacetylases | YJB2475 | RAS2G19V hda1Δ | Sensitive |
| YJB2531 | RAS2G19V hda1Δ YEp55-CAC3 | Resistant | |
| YJB2476 | RAS2G19V rpd3Δ | Sensitive | |
| YJB2530 | RAS2G19V rpd3Δ YEp55-CAC3 | Resistant | |
| YJB2474 | RAS2G19V had1Δrpd3Δ | Sensitive | |
| YJB2532 | RAS2G19V had1Δrpd3Δ YEp55-CAC3 | Resistant | |
| Histone transcription | YJB2998 | RASG19V hir3Δ | Sensitive |
| YJB2999 | RAS2G19V hir3Δ YEp55-CAC3 | Resistant | |
| Resistant | |||
| Silencing maintenance | YJB3763 | RAS2G19V sir3Δ | Sensitive |
| YJB3773 | RAS2G19V sir3Δ YEp55-CAC3 | Resistant | |
| Cell signaling | YJB3670 | RAS2G18A G19V sch9Δ | Sensitive |
| YJB3674 | RAS2G18A G19V sch9Δ YEp55-CAC3 | Resistant | |
| YJB5245 | RAS2G19V gpr1Δ | Sensitive | |
| YJB5237 | RAS2G19V gpr1Δ YEp55-CAC3 | Resistant | |
| YJB5243 | RAS2G19V gpa2Δ | Sensitive | |
| YJB5235 | RAS2G19V gpa2Δ YEp55-CAC3 | Resistant |
a
Sensitive indicates that the heat shock response was indistinguishable from that of an isogenic RAS2G19V strain (YJB2235, -2349, -2474, and -3016). Resistant indicates that the heat shock response was indistinguishable from that of an isogenic RAS2 strain (YJB195, -1583, -2431, and -2136).