Fig 4. Das-1 IgG and IgM specifically recognize 3’-Sulfo-Le^A.

Direct ELISA using Das-1 IgG (A) or Das-1 IgM (C) in the absence or presence of several free glycans in solution at 200 μM. Direct ELISA using Das-1 IgG (B) or Das-1 IgM (D) against a titration series of 3’-Sulfo-Le^A. Data reported as average ± standard deviation of three technical replicates normalized to the reaction without glycans (PBS). Key: Schematic diagram of the relevant Lewis antigens is provided for reference.