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. 2021 Dec 3;17(12):e1010134. doi: 10.1371/journal.ppat.1010134

Fig 5. Effect of actG on total proteins acetylation and in vitro enzymatical acetylation.

Fig 5

(A) The extracted total proteins were analyzed by anti-acetyl lysine Western blotting. The upregulated acetylated protein was identified by mass spectrometry (MS). (B) Coomassie staining, anti-acetyl lysine Western blotting, and zymogram analysis of Gtfs incubated with ActG as acetyltransferase and Ac-CoA as the acetyl donor for 3h at 37°C. The band signals were quantified with Image J software and normalized to the control. Results are presented as mean ± SD (** P <0.01 or *** P < 0.001).