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. 2021 Dec 15;17(12):e1010083. doi: 10.1371/journal.ppat.1010083

Table 2. IC50 valuesa of integrin Ab-, FK-, sITG- and peptide-mediated blocking of MAdV and chimeric HAdV reporter infection.

Cell type / mean fluorescence intensity (MFI) of staining with indicated control and integrin antibodies MOI Blocking Agent b M1-IX-G H5-ΔE3B-CG-FK-M1 M3-IX-G H5-ΔE3B-CG-FK-M3 M2-ΔE1A-G H5-ΔE3B-CG
CMT93 mouse colon cancer /
con Ab: 304
anti-β6: 4,599
anti-β8: 357
1 Ab-β6 124 138 95 119 >800 >800
1 Ab β8 >800 >800 >800 >800 >800
1 FK-M1 4.8 5.6 3.5 5.4 > 800
1 FK-M3 3.6 4.1 2.1 3.5 > 800 > 800
1 FK-H5 >800 >800 >800 >800 >800 22
1 sITG mαvβ3 >800 >800
1 sITG mαvβ6 80 59
1 sITG mαvβ8 >800 >800
1 sITG hαvβ6 83 52
1 sITG hαvβ8 >800 >800
1 A20FMDV2 6.3 4.7 1.1 >5,000
1 A20FMDV2-E >5,000 >5,000 3,770 > 5000
1 A20M1 26 30 5.3 > 5000
1 A20M3 7.4 1.3
B16-mβ6 mouse melanoma /
con Ab: 142
anti-β6: 3,992
anti-β8: 150
3 Ab-β6 150 551 124 201
3 Ab-β8 >800 >800 >800 >800 >800
B16-m β8 /
con Ab: 123
anti-β6: 130
anti-β8: 3,561
3 Ab-β6 >800 >800 >800 >800
3 Ab-β8 61 95 17 75
M000216 human
melanoma /
con Ab: 219
anti-β6: 259
anti-β8: 9,052
3 Ab β6 >800 >800 >800 >800 >800
3 Ab β8 73 120 13 64 >800
3 FK-M1 393 112
3 FK-M3 116 68
3 FK-H5 >800 >800
3 sITG mαvβ3 >800 >800
3 sITG mαvβ6 11 4.5
3 sITG mαvβ8 >800 >800
3 sITG hαvβ6 7.6 2.3
3 sITG hαvβ8 >800 >800
3 A20FMDV2 898 165
3 A20M1 ~5,000 150
3 A20M3 422 17.9
A549-hβ6 human lung carcinoma
con Ab: 219
anti-β6: 1,349
anti-β8: 220
3 A20FMDV2 110
3 A20M1 336

a Data correspond to blocking experiments shown in Figs 2 and 6 and S4, S8, S9 and S11, respectively.

b IC50 concentrations of antibodies, FKs and sITGs are in ng/ml; concentration of peptides are in nM