Table 2. IC50 valuesa of integrin Ab-, FK-, sITG- and peptide-mediated blocking of MAdV and chimeric HAdV reporter infection.
Cell type / mean fluorescence intensity (MFI) of staining with indicated control and integrin antibodies | MOI | Blocking Agent b | M1-IX-G | H5-ΔE3B-CG-FK-M1 | M3-IX-G | H5-ΔE3B-CG-FK-M3 | M2-ΔE1A-G | H5-ΔE3B-CG |
---|---|---|---|---|---|---|---|---|
CMT93 mouse colon cancer / con Ab: 304 anti-β6: 4,599 anti-β8: 357 |
1 | Ab-β6 | 124 | 138 | 95 | 119 | >800 | >800 |
1 | Ab β8 | >800 | >800 | >800 | >800 | >800 | ||
1 | FK-M1 | 4.8 | 5.6 | 3.5 | 5.4 | > 800 | ||
1 | FK-M3 | 3.6 | 4.1 | 2.1 | 3.5 | > 800 | > 800 | |
1 | FK-H5 | >800 | >800 | >800 | >800 | >800 | 22 | |
1 | sITG mαvβ3 | >800 | >800 | |||||
1 | sITG mαvβ6 | 80 | 59 | |||||
1 | sITG mαvβ8 | >800 | >800 | |||||
1 | sITG hαvβ6 | 83 | 52 | |||||
1 | sITG hαvβ8 | >800 | >800 | |||||
1 | A20FMDV2 | 6.3 | 4.7 | 1.1 | >5,000 | |||
1 | A20FMDV2-E | >5,000 | >5,000 | 3,770 | > 5000 | |||
1 | A20M1 | 26 | 30 | 5.3 | > 5000 | |||
1 | A20M3 | 7.4 | 1.3 | |||||
B16-mβ6 mouse melanoma / con Ab: 142 anti-β6: 3,992 anti-β8: 150 |
3 | Ab-β6 | 150 | 551 | 124 | 201 | ||
3 | Ab-β8 | >800 | >800 | >800 | >800 | >800 | ||
B16-m β8 / con Ab: 123 anti-β6: 130 anti-β8: 3,561 |
3 | Ab-β6 | >800 | >800 | >800 | >800 | ||
3 | Ab-β8 | 61 | 95 | 17 | 75 | |||
M000216 human melanoma / con Ab: 219 anti-β6: 259 anti-β8: 9,052 |
3 | Ab β6 | >800 | >800 | >800 | >800 | >800 | |
3 | Ab β8 | 73 | 120 | 13 | 64 | >800 | ||
3 | FK-M1 | 393 | 112 | |||||
3 | FK-M3 | 116 | 68 | |||||
3 | FK-H5 | >800 | >800 | |||||
3 | sITG mαvβ3 | >800 | >800 | |||||
3 | sITG mαvβ6 | 11 | 4.5 | |||||
3 | sITG mαvβ8 | >800 | >800 | |||||
3 | sITG hαvβ6 | 7.6 | 2.3 | |||||
3 | sITG hαvβ8 | >800 | >800 | |||||
3 | A20FMDV2 | 898 | 165 | |||||
3 | A20M1 | ~5,000 | 150 | |||||
3 | A20M3 | 422 | 17.9 | |||||
A549-hβ6 human lung carcinoma con Ab: 219 anti-β6: 1,349 anti-β8: 220 |
3 | A20FMDV2 | 110 | |||||
3 | A20M1 | 336 | ||||||