Table 4. SPR kinetic data of soluble integrin binding to immobilized FK-M1 and FK-M3.
| FK Probe | Analyte | Surface density (response units) (RU) | kon (104 M-1s-1) | koff (s-1) | KD (nM) | Chi2 (RU2) |
|---|---|---|---|---|---|---|
| M1 | mαvβ6 | 130 | 6.64 | 1.66x10-5 | 0.250 a | 0.01 |
| M3 | mαvβ6 | 160 | 5.13 | 3.12x10-8 | < 0.01 b | 0.29 |
| M1 | hαvβ6 | 130 | 8.02 | 1.27x10-4 | 1.58 c | 0.01 |
| M3 | hαvβ6 | 160 | 8.50 | 5.01x10-6 | 0.059 d | 0.02 |
| M1 | hαvβ8 | 3130 | 46.5 | 6.73x10-3 | 14.5 e | 0.02 |
| M3 | hαvβ8 | 1340 | 72.9 | 9.65x10-4 | 1.32 f | 0.26 |
a data corresponds to experiment shown in Fig 7A
b data corresponds to experiment shown in Fig 7B
c data corresponds to experiment shown in S12A Fig
d data corresponds to experiment shown in S12B Fig
e data corresponds to experiment shown in S12C Fig
f data corresponds to experiment shown in S12D Fig