Fig 5. Bfc interacts with Srp to regulate crq expression and efferocytosis.

A. The relative crq mRNA levels were quantified by qPCR in macrophages obtained from wild-type, homozygous bfc^ko or srp³, and double heterozygous bfc^ko and srp³ stage 13 embryos. B. Western blot analysis of the Crq protein levels in macrophages of wild-type, homozygous bfc^ko or srp³, and double heterozygous bfc^ko and srp³ stage 13 embryos. Actin was used as the loading control. C. Quantification of the Crq protein levels in (B) after normalization to the Actin levels; n = 3. D. Macrophages of wild-type, homozygous bfc^ko or srp³, and double heterozygous bfc^ko and srp³ stage 13 embryos were stained with anti-Crq (green) and anti-Dcp1 (magenta) antibodies. Scale bar = 20 μm. E. Graph showing the mean PIs ± SEM for each genotype in (D). F. The relative crq mRNA levels were quantified by qPCR in macrophages of wild-type, homozygous bfc^ko or crq^ko, and double heterozygous bfc^ko and crq^ko stage 13 embryos. G. Western blot analysis of the Crq protein levels in macrophages of wild-type, homozygous bfc^ko or crq^ko, and double heterozygous bfc^ko and crq^ko stage 13 embryos. Actin was used as the loading control. H. Quantification of the Crq protein levels in (G) after normalization to the Actin levels; n = 3. I. Macrophages of wild-type, homozygous bfc^ko or crq^ko, and double heterozygous bfc^ko and crq^ko stage 13 embryos were stained with anti-Crq (green) and anti-Dcp1 (magenta) antibodies. Scale bar = 20 μm. J. Graph showing the mean PIs ± SEM for each genotype in (I). Statistical significance was assessed using the one-way ANOVA.