TABLE 1.
Capture probes and primers for emm typing of S. pyogenes
| Serotype or primer designation | Label and sequence |
|---|---|
| Serotype | |
| M, all | Biotin-TTAGTTTWGCAAGTTCTTCAGCTTGTTT |
| M1 | Biotin-TTCTATAACTTCCCTAGGATTACCATC |
| M3 | Biotin-ATGTCTAGGAAACTCTCCATTAACACT |
| M4 | Biotin-GAATCAGCCTGAGGCTTTTTAATCTC |
| M5 | Biotin-CGGGTCATTTATTGTACCCCTAGTC |
| M6 | Biotin-GCTTTGTCCGGGTTTTCTACCGTC |
| M9 | Biotin-GGCGGAAGGGGTTAAGAAGGCGGAA |
| M11 | Biotin-AGCGCTTTGCCCCGCAGCCTTAA |
| M12 | Biotin-GTAGAGTTCTGAACGCTGTTTCAG |
| M13 | Biotin-CCAGAAACAGAACAGCGTTTCTAATGA |
| M22 | Biotin-TTATTAGTTTGCTTTCTTGAGAAAT |
| M28 | Biotin-AAGTCTCAGTACTTTTTGGAGACTCC |
| M58 | Biotin-GCTTTAGCCTTTGCACTATCCGCC |
| M76 | Biotin-TTAGAAACGCTTTTAGAGTTCGCGTC |
| M77 | Biotin-GGTCTGTAATGCGGTTATGTAAGTGAT |
| M78 | Biotin-TTAGTAATACTACGAGAGTTCTGAGAC |
| PT2233 | Biotin-TCGTTTTGATCTAGCAAGTCTTGCA |
| PT4245 | Biotin-ATTCTTTTTCATTATCTTTGACAGA |
| PT4931 | Biotin-TCGATACCTTCTTCTCCAAGTTATTCT |
| Primers | |
| MF | Fluorescein-ATAAGGAGCATAAAAATGGCT |
| MR | AGCTTAGTTTTCTTCTTTGCG |