FIGURE 6.

SCI-101 treatment augments glioblastoma susceptibility to GTA002-mediated killing. (A) U-87 or U-251 cells pre-treated for 72 h with 5 μM SCI-101 or left untreated were co-cultured with GTA002 cells at an E:T ratio of 1:1 for 60 h in Incucyte. (B, C) Cell proliferation traces normalized to time point 0 h and to the relative viability of U-251 (B) and U-287 (C) are shown as the mean $\pm$ SEM of technical quadruplicates for one GTA002 donor. (D, E) Representative images of U-251 (D) and U-287 (E) at t=60 h. Tumor cells are shown with red nuclei. (F, G) Statistical analysis of relative viability was performed using the nested t-test at t=60 h for U-251 (F, p=0.0029) and U-287 (G, p=0.0078). Data are shown as the mean $\pm$ SEM of n=3 GTA002 donors with ** p<0.01.