Table 3.
Oligonucleotides used in this study
| Primer | Purpose | Sequence1 |
|---|---|---|
| 2089 | Amplify AbR cassette, F | CCATACTTAGTGCGGCCGCCTA |
| 2090 | Amplify AbR cassette, R | CCATGGCCTTCTAGGCCTATCC |
| 2416 | Delete hfq, upstream | CGTTGGAAGTATTTCGAATTTCG |
| 2417 | Delete hfq, upstream | taggcggccgcactaagtatggGCTATGAGCCTTTAGCTTATGAC |
| 2418 | Delete hfq, downstream | ggataggcctagaaggccatggCTAGAATAGTTAATCAGTTAAGG |
| 2419 | Delete hfq, downstream | GCTTTAATACGTTCACGCAACAATCG |
| 2420 | Complement hfq | ggataggcctagaaggccatggGCTTCAATGTTGTTTAACTTAAGTGC |
| 2421 | Complement hfq | taggcggccgcactaagtatggCCTTAACTGATTAACTATTCTAG |
| 2290 | Amplify from middle of ErmR | AAGAAACCGATACCGTTTACG |
| 1487 | Amplify glmS | GGTCGTGGGGAGTTTTATCC |
| 2196 | Amplify glmS | tCCATACTTAGTGCGGCCGCCTA |
| 2424 | Delete qrr1, upstream | GGTATCTTTTGGATTCTCTTGG |
| 2425 | Delete qrr1, upstream | taggcggccgcactaagtatggCCTATTGCAGGGAGCGTGCCAAC |
| 2426 | Delete qrr1, downstream | ggataggcctagaaggccatggGCTATAAAATCAATAACTAACTATTC |
| 2427 | Delete qrr1, downstream | CGCTTAGGTGAGTTTGATGTCC |
| 2242 | 5S rRNA, qRT-PCR, F | TGATCCCATGCCGAACTCAGAAG |
| 2243 | 5S rRNA, qRT-PCR, R | CCTGGCGATGTCCTACTCTCAC |
| qlitR-F | litR, qRT-PCR, F | AAGGCCTAGAACAAGGCTATC |
| qlitR-R | litR, qRT-PCR, R | GACAGAGACCTGAGCGATTT |
1
Lowercase letters indicate “tail” sequences not complementary to the template.