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. 2021 Dec 15;7(51):eabj1281. doi: 10.1126/sciadv.abj1281

Fig. 6. Analysis of XPRIZE contrived sample panel without RNA extraction.

Fig. 6.

(A) Viral particles spiked in 1× PBS were tested in triplicate for each sample (n = 9 total for positive sample concentrations and n = 6 total for negative samples). (B) Viral particles spiked in human nasal matrix were tested in triplicate (n = 6 total for each concentration). (C) Viral particles spiked in saliva (n = 6 total for each concentration). (D) Summary table for the sensitivity and specificity of the Harmony COVID-19 system on the XPRIZE contrived samples. Concentrations of viral particles are the final concentrations in each 40-μl reaction. Threshold concentration for positive is at 20 viral particle copies per reaction (0.5 copies/μl). Contrived samples below the positive threshold were also tested (fig. S7). Real-time amplification curves of these data are in fig. S8. Reactions were set up either at room temperature (hollow) or on ice (solid).