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. 2021 Nov 15;10:e70412. doi: 10.7554/eLife.70412

Figure 2. ZHX2 is essential for triple-negative breast cancer (TNBC) cell proliferation and invasion.

(A–H) Immunoblot of cell lysates (A), Real-time quantitative PCR (qRT-PCR) (B), cell proliferation assays (C), 2D colony formation assays (D), representative images of 3D soft agar growth (E) and quantification of oft agar colonies (F), invasion assay (G), and quantification of invasion assay (H) of MDA-MB-231/468 cells infected with lentivirus encoding two individual ZHX2 shRNAs (43 and 45) or control shRNA (Ctrl). (I–N) Immunoblot of cell lysates (I), cell proliferation (J), 2D colony formation assay (K), representative images of 3D soft agar growth (upper), and invasion assays (down) (L) as well as their quantifications (M, N) of MDA-MB-231 cells infected with lentivirus encoding sh45-resistant ZHX2 (ZHX2res) or control vector (EV), then followed by ZHX2 sh45 or Ctrl lentivirus infection. Error bars represent mean ± standard error of the mean (SEM), unpaired t-test. **p < 0.01; ***p < 0.001.

Figure 2—source data 1. Uncropped western blot images for Figure 2.

Figure 2.

Figure 2—figure supplement 1. The phenotype of ZHX2 shRNA on cell proliferation and invasion is due to its on-target effect.

Figure 2—figure supplement 1.

(A–E) Immunoblot of cell lysates (A), cell proliferation assays (B), representative 2D colony formation (C), representative soft agar colony (D), quantification of soft agar colony (E), invasion assays (F), and quantification of invasion assays (G) of MDA-MB-468 cells transfected with ZHX2 sh45-resistant (ZHX2res) or empty vector (EV), followed by ZHX2 sh45 or control (Ctrl) shRNA infection. Error bars represent mean ± standard error of the mean (SEM), unpaired t-test. *p < 0.05; **p < 0.01; ***p < 0.001.
Figure 2—figure supplement 1—source data 1. Uncropped western blot images for Figure 2—figure supplement 1.