| Gene (Homo sapiens) |
ZHX2
|
GenBank |
Gene ID: 22,882 |
|
| Cell line (Homo sapiens) |
MDA-MB-231 |
ATCC |
Catalog # ATCC HTB-26RRID:CVCL_0062
|
|
| Cell line (Homo sapiens) |
MDA-MB-468 |
ATCC |
Catalog # ATCC HTB-132RRID:CVCL_0419
|
|
| Antibody |
Rabbit anti ZHX2 antibody(Rabbit Polyclonal) |
Genetex |
Catalog # 112,232RRID:AB_10727176
|
WB (1:1000)Co-IP (1:100)IHC (1:500) |
| Antibody |
Rabbit anti HIF1α(Rabbit Monoclonal) |
Cell Signaling |
Catalog # 36,169RRID:AB_2861751
|
WB (1:1000) |
| Antibody |
Rabbit anti VHL(Rabbit Polyclonal) |
Cell Signaling |
Catalog # 68,547 |
WB (1:1000)IHC (1:200) |
| Recombinant DNA reagent |
pLKO.1 |
Addgene |
Catalog # 10,878RRID:Addgene_10878
|
TRC cloning vector |
| Recombinant DNA reagent |
lentiCRISPR v2 |
Addgene |
Catalog # 52,961RRID:Addgene_52961
|
|
| Recombinant DNA reagent |
pcDNA3.1 |
Addgene |
Catalog # 52,535RRID:Addgene_52535
|
|
| Chemical compound, drug |
MG132 |
Peptide International |
Catalog # IZL-3175-v |
|
| Chemical compound, drug |
Doxycycline hydrochloride |
Sigma-Aldrich |
Catalog # D3447 |
|
| Chemical compound, drug |
Agarose |
Life Technologies |
Catalog # BP165-25 |
Soft agar assay |
| Commercial assay or kit |
MTS Assay Kit (Colorimetric) |
Abcam |
Catalog # ab197010 |
Cell proliferation assay |
| Commercial assay or kit |
BD BioCoat Matrigel Invasion Chamber |
Corning |
Catalog # ab354480 |
Invasion assay |
