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. 2021 Dec 1;10:e73348. doi: 10.7554/eLife.73348

Figure 7. MCPH1 has little effect on condensin II ATP hydrolysis and DNA binding.

Figure 7.

(A) ATPase rate of condensin II complex in the presence of MCPH1. Q refers to condensin II with an ATPase deficient mutation in the Q-loop. Below is an SDS page gel of the completed reaction. Error bars indicate standard deviation from three repeats. (B) EMSA assay of MBP, MCPH11-435-MBP and -MCPH1195-435-MBP using 50 bp of Cy5-labelled dsDNA. (C) EMSA assay of condensin II in the presence of MBP or MCPH11-435-MBP. (D) EMSA assay of condensin II in the presence or absence of 5FAM-MCPH1 peptide. Top image detecting Cy5 and bottom imaged detecting 5FAM.

Figure 7—source data 1. Microsoft excel data corresponding to the standard curve in Figure 7A.
Figure 7—source data 2. Microsoft excel data corresponding to the ATPase curve in Figure 7A.
Figure 7—source data 3. raw data uncropped gels corresponding to Figure 7.