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. 2021 Dec 1;10:e73348. doi: 10.7554/eLife.73348
Targeted Gene sgRNA
Ncaph2-Gfp GGTGGAAAGTAGTATATACC
Ncaph2-Halo GGTGGAAAGTAGTATATACC
Mcph1 deletion Sg5' GGTGTGCAATTCCTAGTGTG
Mcph1 deletion Sg3' AGCTGTTCCTTAGAACACGA
Mcph1-Gfp ACAGTGAGACATCTACAATG
Stop-Tev CATGGATTTCTCCGGTGAAT
Wapl-Lox-Tev Sg5' AATGGGTGCTTATAATTAGC
Wapl-Lox-Tev Sg3' ACAATGTCACAATGGCTCAT
Scc1-Halo ATAATATGGAACCGTGGTCC
DelCen-Mcph1 cgttgaggcttcttcctatg
TEV sites in WaplTwo Guide RNA used in combination AATGGGTGCTTATAATTAGCACAATGTCACAATGGCTCAT