Targeted Gene | sgRNA |
---|---|
Ncaph2-Gfp | GGTGGAAAGTAGTATATACC |
Ncaph2-Halo | GGTGGAAAGTAGTATATACC |
Mcph1 deletion Sg5' | GGTGTGCAATTCCTAGTGTG |
Mcph1 deletion Sg3' | AGCTGTTCCTTAGAACACGA |
Mcph1-Gfp | ACAGTGAGACATCTACAATG |
Stop-Tev | CATGGATTTCTCCGGTGAAT |
Wapl-Lox-Tev Sg5' | AATGGGTGCTTATAATTAGC |
Wapl-Lox-Tev Sg3' | ACAATGTCACAATGGCTCAT |
Scc1-Halo | ATAATATGGAACCGTGGTCC |
DelCen-Mcph1 | cgttgaggcttcttcctatg |
TEV sites in WaplTwo Guide RNA used in combination | AATGGGTGCTTATAATTAGCACAATGTCACAATGGCTCAT |