| Strain, strain background (Mus musculus) |
BmxCreER
|
Mouse Genome Informatics |
MGI: 5513853; RRID: IMSR_TAC:14081
|
|
| Strain, strain background (Mus musculus) |
RosatdTomato
|
The Jackson Laboratory |
Stock No: 007909; RRID: IMSR_JAX:007909
|
|
| Strain, strain background (Mus musculus) |
CD1 |
Charles River Laboratories |
Strain code: 022; RRID: IMSR_CRL:022
|
|
| Biological sample (Homo sapiens) |
Primary fetal heart tissue |
Collected at Stanford from elective terminations |
|
|
| Antibody |
Rat anti- APC/Cy7 Cd45 (rat monoclonal) |
Biolegend |
Cat #: 103116; RRID: AB_312981
|
(1:50) |
| Antibody |
Rat anti- APC Pecam1 (rat monoclonal) |
Biolegend |
Cat #: 102410; RRID: AB_312905
|
(1:50) |
| Antibody |
Rat anti- APC-Cy7 Ter119 (rat monoclonal) |
Biolegend |
Cat #: 116223; RRID: AB_2137788
|
(1:50) |
| Antibody |
Mouse anti- Pacific Blue CD235a (mouse monoclonal) |
Biolegend |
Cat #: 349107; RRID: AB_11219199
|
(1:50) |
| Antibody |
Mouse anti- FITC CD36 (mouse monoclonal) |
Biolegend |
Cat #: 336204; RRID: AB_1575025
|
(1:50) |
| Antibody |
Mouse anti- APC-Cy7 PECAM1 (mouse monoclonal) |
Biolegend |
Cat #: 303119; RRID: AB_10643590
|
(1:50) |
| Antibody |
Mouse anti- Pacific Blue CD45 (mouse monoclonal) |
Biolegend |
Cat #: 304021; RRID: AB_493654
|
(1:50) |
| Antibody |
Mouse anti- PerCP-Cy5.5 CD235a (mouse monoclonal) |
Biolegend |
Cat #: 349110; RRID: AB_2562706
|
(1:50) |
| Antibody |
Mouse anti-PerCP-Cy5.5 CD45 (mouse monoclonal) |
Biolegend |
Cat #: 304028; RRID: AB_893338
|
(1:50) |
| Antibody |
Rabbit anti-Erg (rabbit monoclonal) |
Abcam |
Cat #: ab92513; RRID: AB_2630401
|
(1:200) |
| Antibody |
Goat anti-Car4 (goat polyclonal) |
R&D |
Cat #: AF2414; RRID: AB_2070332
|
(1:200) |
| Antibody |
Rabbit anti-Smmhc (rabbit polyclonal) |
Proteintech |
Cat #: 21404–1-AP; RRID: AB_10732819
|
(1:100) |
| Antibody |
Mouse anti-Cldn5 (mouse monoclonal) |
Invitrogen |
Cat #: 35–2500; RRID: AB_2533200
|
(1:200) |
| Peptide, recombinant protein |
Collagenase IV |
Worthington |
Cat #: LS004186
|
|
| Peptide, recombinant protein |
Dispase |
Worthington |
Cat #: LS02100 |
|
| Peptide, recombinant protein |
DNase I |
Worthington |
Cat #: LS002007
|
|
| Commercial assay or kit |
RNEasy Mini Kit |
Qiagen |
Cat #: 74,104 |
|
| Commercial assay or kit |
iScript Reverse Transcription Supermix |
Bio-Rad |
Cat #: 1708840 |
|
| Commercial assay or kit |
Roche DIG RNA Labeling kit |
Millipore Sigma |
Cat #: 11175025910 |
|
| Commercial assay or kit |
RNAscope Multiplex Fluorescent V2 assay kit |
Advanced Cell Diagnostics |
Cat #: 323,100 |
|
| Commercial assay or kit |
Click-iT EdU Imaging kit |
Thermo Fisher Scientific |
Cat #: C10086
|
|
| Chemical compound, drug |
Tamoxifen |
Sigma-Aldrich |
Cat #: T5648 |
|
| Chemical compound, drug |
4-OH Tamoxifen |
Sigma-Aldrich |
Cat #: H6278 |
|
| Software, algorithm |
Seurat v3 |
https://doi.org/10.1016/j.cell.2019.05.031
|
RRID: SCR_007322
|
|
| Software, algorithm |
bcl2fastq |
illumina |
RRID: SCR_015058
|
|
| Software, algorithm |
cutadapt 2.7 |
https://doi.org/10.14806/ej.17.1.200
|
RRID: SCR_011841
|
|
| Software, algorithm |
Cell Ranger v3.1.0 |
10× Genomics |
RRID: SCR_017344
|
|
| Software, algorithm |
STAR v2.7.1a |
https://doi.org/10.1093/bioinformatics/bts635
|
RRID: SCR_004463
|
|
| Software, algorithm |
Subread v1.6.0 |
https://doi.org/10.1093/nar/gkt214
|
RRID: SCR_009803
|
|
| Software, algorithm |
heatmaply |
http://dx.doi.org/10.1093/bioinformatics/btx657
|
|
https://github.com/talgalili/heatmaply (Galili, 2021) |
| Software, algorithm |
biomaRt |
10.18129/B9.bioc.biomaRt
|
RRID: SCR_019214
|
|
| Software, algorithm |
PAGA |
https://doi.org/10.1186/s13059-019-1663-x
|
|
https://github.com/theislab/paga (Thesis Lab, 2019) |
| Software, algorithm |
RNA velocity |
10.1038/s41586-018-0414-6
|
RRID: SCR_018168; RRID: SCR_018167
|
|
| Software, algorithm |
Slingshot |
https://doi.org/10.1186/s12864-018-4772-0
|
RRID: SCR_017012
|
|
| Software, algorithm |
SCENIC |
10.1038/nmeth.4463
|
RRID: SCR_017247
|
|
| Software, algorithm |
FIJI |
doi:10.1038/nmeth.2019
|
RRID: SCR_002285
|
|
| Software, algorithm |
QuPath |
https://doi.org/10.1038/s41598-017-17204-5
|
RRID: SCR_018257
|
|
| Software, algorithm |
Prism 8 |
GraphPad Software |
RRID: SCR_002798
|
|
| Other |
TINAGL1 RNA probe |
Advanced Cell Diagnostics |
Cat #: 857221-C2 |
|
