Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 2;12:744408. doi: 10.3389/fmicb.2021.744408

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Shen, Li, Cheng, Wang, Wu, Yang, Jia, Tian, Ou, Mao, Sun, Zhang, Zhu, Chen, Liu, Zhao, Huang, Gao, Liu, Yu, Zhang and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The influence of LORF5 on virus adsorption, invasion, and replication. ΔLORF5, RΔLORF5, and CHv-BAC (0.001 MOI) were inoculated into DEF cells. On the one hand, the number of virus plaques was calculated to study the ability to adsorb on the cell surface (A) or invade cells (C) after 24 hpi; on the other hand, cell samples were collected 0–2 h after incubating viruses, and the copies were separately detected simultaneously (B,D). (E) Cell samples infected with ΔLORF5, RΔLORF5, and CHv-BAC (1 MOI) were collected, RNAs were extracted and reverse-transcribed into cDNA as a template to detect the virus copy number by q-PCR (t-test, ^∗P < 0.05).