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. 2021 Dec 2;12:744408. doi: 10.3389/fmicb.2021.744408

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Copyright © 2021 Shen, Li, Cheng, Wang, Wu, Yang, Jia, Tian, Ou, Mao, Sun, Zhang, Zhu, Chen, Liu, Zhao, Huang, Gao, Liu, Yu, Zhang and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The role of LORF5 in the assembly and release of virus particles. (A) Electron microscopy analysis of DEF cells infected with CHv-BAC-ΔLORF5. DEF cells were infected with 5 MOI of virus and examined by electron microscopy analysis (N, nucleus; C, cytoplasm). The white box in the middle indicates virus particles. (B) Eighteen hours after the cells were infected with the viruses, the medium was replaced with 2% maintenance solution; the supernatant was collected at 30, 60, 90, and 120 min after changing; and the infectious mature virus particles in the supernatant were detected. (C) At the same time, the cells were used as control. The means and standard deviations were measured with GraphPad Prism 8. Standard deviations are shown by error bars.