TABLE 3.

Primers used in this paper.

Name	Sequence (5′–3′)	Product
ΔLORF5-Kan-F	cgtactaactgaagttgaatctgatcta gtaaccggggcctagggataacagggta atcgatttatgtgaaagacgtcaaaagttaaa accggtatattaaatgggccccggtt	Target ΔLORF5 fragment
ΔLORF5-Kan-R	actagatcagattcaacttcagttagtacggcca gtgttacaaccaat
ΔLORF5-F	gccaacggtagggactg	ΔLORF5/RΔLORF5 identification
ΔLORF5-R	gacacggtaaacaatgaagg
RΔLORF5-F	aacagatgtccgtatgtaatcgtactaactg aagttgaatctgatctagtaaccg gggccatggcatcttcaaaagcgtt	Target RΔLORF5 fragment
RΔLORF5-R	aaagttaaaaccggtatattaaatgctaata gtcatctctggtat
RΔLORF5-Kan-F	catttaatataccggtttt aacttttgacgtctttcacattacttaaatat agggataacagggtaatcgat
RΔLORF5-Kan-R	cgagactgattcgtttaatcatatttatttt atttaagtaatgtgaaagacgtcaaaagtt aaaaccggtatattaaatgt gttacaaccaattaacc
US8-F	tctcaagacgctctggaatc	US8 (gE)
US8-R	gacgcagagaagtactcgct
LORF5-F	aacgactggtgggagtaacg	LORF5
LORF5-R	gcagcggaacaaatgaaac
LORF4-F	cgcaccctatgctatcgtc	LORF4
LORF4-R	cgttgtcggattacccattt
UL55-F	tattcttctgcgggctca	UL55
UL55-R	catagacgatgctcc
UL30-F	tttcctcctcctcgctgagtg	UL30
UL30-R	ccagaaacatactgtgagagt