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Experimental parameters for Capflex analysis of RP3 on the FIDA 1 instrument.
| Tray | Vial | Pressure (mbar) | Time (s) | Outlet | Measure | Comment |
|---|---|---|---|---|---|---|
| 2 | 1 | 3500 | 40 | Variable | No | 300 mM NaCl wash |
| 2 | 2 | 3500 | 40 | Variable | No | milliQ wash |
| 1 | Indicator | 3500 | 40 | Variable | No | Buffer wash |
| 1 | Analyte | 400 | 300 | Variable | Yes | Sample application |
Trays 1 and 2 were maintained at 20 °C and the capillary chamber was also maintained at 20 °C.
